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면역학 및 감염병학

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一个简单的的Chelex协议对DNA的提取<em>按蚊</em>属。

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

A Simple Chelex Protocol for DNA Extraction from Anopheles spp.

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一个简单的的Chelex协议对DNA的提取<em>按蚊</em>属。

53,386 Views

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07:16 min

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January 09, 2013

DOI:

DOI:

•

07:16 min

January 09, 2013

•

31 Views

Mulenga Musapa¹, Taida Kumwenda¹, Mtawa Mkulama¹, Sandra Chishimba¹, Douglas E. Norris², Philip E. Thuma¹, Sungano Mharakurwa¹

¹Malaria Institute at Macha, ²Department of Molecular Microbiology & Immunology,Johns Hopkins Bloomberg School of Public Health

Summary

一种快速，经济实惠的方式来提取质量疟疾寄生虫的蚊子标本与载体DNA的方法。利用Chelex树脂螯合性能，简单的方法使基因分型疟疾寄生虫的蚊子中肠和唾液腺阶段，以及分子鉴定<em>按蚊</em近缘种PCR。

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