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果蝇蛹腹部免疫
JoVE 신문
생물학
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JoVE 신문 생물학
Drosophila Pupal Abdomen Immunohistochemistry
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果蝇蛹腹部免疫

DOI:
10.3791/3139-v

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08:29 min

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October 02, 2011

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,
1Department of Biological Sciences,University of Alabama

Chapters

  • 00:05Title
  • 01:19Collection, Culturing and Staging Pupae
  • 02:33Dissection
  • 03:40Cleaning, Fixation and Primary Antibody Incubation
  • 05:38Secondary Antibody Incubation and Mounting
  • 07:24Images of Prepared Samples
  • 08:00Conclusion

Summary

자동 번역

자동 번역

抗体染色<em>果蝇</em>蛹可以提高成人腹部发育遗传学的遗传分析。我们目前对我们的协议清扫，固定和抗体染色上演<em>果蝇</em>蛹腹部。

Tags

DrosophilaPupal AbdomenImmunohistochemistryEpithelial MorphogenesisSexually Dimorphic MorphologiesImaginal CellsHistoblast NestsLarval EpidermisAdult Abdominal SegmentsDorsal CuticleVentral CuticleSpiraclesDiploid CellsLarval Epidermal CellsMolecular ToolsGenetic PathwaysAdult PhenotypesDissectionImmunohistochemical Analyses

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