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一种高通量自动化的平台,制造蛋白质治疗的细胞系发展
JoVE 신문
의학
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JoVE 신문 의학
A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
DOI:

07:48 min

September 22, 2011

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Chapters

  • 00:05Title
  • 01:16Host Cell Transfection
  • 03:02Cloning by Flow Cytometry Activated Cell Sorting
  • 04:38Screening and Selection of Highly Productive Clones
  • 05:52Analysis of Antibody Producing Clones
  • 07:29Conclusion

Summary

자동 번역

描述的是一种高通量,自动化平台生产蛋白质药物的制造细胞系的发展。 BD流式细胞仪咏叹调细胞分选的实施,CloneSelect成像和TECAN自由EVO液体处理系统已显示出显着增加细胞系的发展与提高细胞的质量和高重现性的加工能力。

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