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Un metodo semplice ed efficace per isolare i macrofagi da Mixed colture primarie di cellule del fegato degli adulti
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Un metodo semplice ed efficace per isolare i macrofagi da Mixed colture primarie di cellule del fegato degli adulti
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May 24, 2011
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Isolate MacrophagesMixed Primary CulturesAdult Liver CellsKupffer CellsLiver-specific Resident MacrophagesIsolation MethodsSophisticated EquipmentCentrifugal ElutriatorTechnical SkillsNovel MethodSufficient Number And PurityRat Liver CellsTwo-step Perfusion MethodParenchymal HepatocytesCulture MediumDMEMFCSEpithelial Cell MorphologyFibroblast-like CellsPhase Contrast-bright Round Macrophage-like CellsProliferation Of Macrophage-like CellsShaking Of Culture FlasksSuspension Of MacrophagesTransfer And Short Incubation In Plastic Dishes
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Kitani, H., Takenouchi, T., Sato, M., Yoshioka, M., Yamanaka, N. A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells. J. Vis. Exp. (51), e2757, doi:10.3791/2757 (2011).
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