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羧基荧光素二醋酸琥珀酰亚胺酯(CFSE)用于监视淋巴细胞增殖
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation

羧基荧光素二醋酸琥珀酰亚胺酯(CFSE)用于监视淋巴细胞增殖

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04:10 min

October 12, 2010

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04:10 min
October 12, 2010

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Summary

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CFSE共价键的标签与荧光染料,羧基的长寿命的细胞内分子。因此,一个CFSE标记的细胞分裂时,它的后代有一半的荧光量,从而可以被用来评估细胞分裂。本文介绍与CFSE标记的小鼠淋巴细胞通常使用的程序。

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