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In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
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In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

DOI:
10.3791/2103-v

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08:24 min

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October 08, 2010

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, , , ,
1Center for Neurologic Diseases,Brigham and Woman’s Hospital and Harvard Medical School, 2Department of Physiology and Neurobiology,University of Connecticut

Chapters

  • 00:00Title
  • 00:21Supscription Lock
  • 00:56Introduction
  • 01:49Injecting DNA and Electroporation
  • 05:36Analyzing Neuronal Process Outgrowth
  • 06:22Analysis of the Cell Types of Electroporated Neurons
  • 07:50Conclusion

Summary

자동 번역

자동 번역

In utero electroporation is a valuable method for transfecting neuronal progenitor cells in vivo. Depending upon the placement of the electrodes and the developmental timepoint of electroporation, certain subsets of cortical cells can be targeted. Targeted cells can then be analyzed in vivo or in vitro for effects of genetic alteration.

Tags

In Utero ElectroporationPrimary Neuronal CultureGene FunctionSubset Of Cortical NeuronsNeurite OutgrowthGenetic AlterationsShRNACDNA ConstructsChemical TransfectionViral TransductionHeterogeneous Pool Of Cell TypesGlutamatergic NeuronsInhibitory NeuronsGlial CellsEmbryonic Rodent CortexDeep Layers Of The CortexSuperficial Layers Of The CortexDorsal-medial Regions Of The CortexVentral-lateral Regions Of The CortexQuantitative AnalysisStep-by-step Method

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