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Visualización de la célula a célula de Transmisión del VIH utilizando clones fluorescente del VIH y la microscopía confocal en vivo
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Chapters
- 00:00Title
- 00:55Introduction
- 01:26Preparing the Donor (Jurkat) and Target (Primary) CD4+ T cells
- 03:47Cleaning Up the Donor Cells and Labeling the Target Cells
- 06:36Monitor Cell-to-cell Transfer of HIV Gag-iGFP Using Live Cell Transfer
- 12:02Representative Images of Cell-to-cell Transfer of HIV
- 12:29Conclusion
Tags
VisualizingCell-to-cell TransferHIVFluorescent ClonesLive Confocal MicroscopyGreen Fluorescent ProteinComplex Cellular ProcessesFluorescence Video MicroscopyHuman Immunodeficiency Virus Core ProteinGag ProteinInfectious Molecular CloneHIV Gag-iGFPVideo Confocal MicroscopyTransfectHuman T Cell LineFluorescently Labeled Uninfected CD4+ T CellsTarget CellsViral ProductionTransportIntercellular StructuresVirological SynapsesGas Permeable Imaging Chambers
