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Establishment of a 2D Monolayer from 3D Bovine Ileal Enteroid Culture
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2D Sığır İleal Enteroid Kaynaklı Tek Tabakanın Epitelyal Bariyer Bütünlüğü ve Birleşmesinin Kantitatif Ölçümü
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Quantitative Measurement of Epithelial Barrier Integrity and Confluency of 2D Bovine Ileal Enteroid-Derived Monolayer
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2D Sığır İleal Enteroid Kaynaklı Tek Tabakanın Epitelyal Bariyer Bütünlüğü ve Birleşmesinin Kantitatif Ölçümü

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01:46 min

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April 05, 2024

DOI:

10.3791/200826-v

DOI:
10.3791/200826-v

•

01:46 min
April 05, 2024

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1 Views
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1Department of Farm Animal Medicine, College of Veterinary Medicine,Midwestern University

Tags

Epithelial BarrierEnteroid MonolayerTransepithelial Electrical Resistance (TEER)Confluency2D CultureBovine IlealConfocal MicroscopyCell DifferentiationEnteroendocrine CellsPaneth CellsEnterocytesCytokine Production

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Quantitative Measurement of Epithelial Barrier Integrity and Confluency of 2D Bovine Ileal Enteroid-Derived Monolayer. J. Vis. Exp. (Pending Publication), e200826, doi:10.3791/200826 (2024).

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