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Establishment of a 2D Monolayer from 3D Bovine Ileal Enteroid Culture
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Geração de Monocamadas 2D a partir de Enteróides Ileais Bovinos 3D
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Generation of 2D Monolayers from 3D Bovine Ileal Enteroids

Geração de Monocamadas 2D a partir de Enteróides Ileais Bovinos 3D

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04:32 min

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April 05, 2024

DOI:

10.3791/200825-v

DOI:
10.3791/200825-v

•

04:32 min
April 05, 2024

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1 Views
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1Department of Farm Animal Medicine, College of Veterinary Medicine,Midwestern University

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2D Monolayers3D Bovine Ileal EnteroidsBasement Membrane Extracellular MatrixBMECell CultureCell DissociationCell SuspensionEnteroid FragmentsEnteroid Growth MediaFBS Wash MediaOrganoid Growth MediaTrypLE Express EnzymeTrypan Blue Dye Exclusion

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Generation of 2D Monolayers from 3D Bovine Ileal Enteroids. J. Vis. Exp. (Pending Publication), e200825, doi:10.3791/200825 (2024).

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