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Generierung von DNA-Sonden zur Markierung der Geschlechtschromosomen von Anopheles-Mücken und Herstellung der Hybridisierungslösung

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Generating DNA Probes to Label the Sex Chromosomes of Anopheles Mosquitoes and Preparing the Hybridization Solution

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Generierung von DNA-Sonden zur Markierung der Geschlechtschromosomen von Anopheles-Mücken und Herstellung der Hybridisierungslösung

150 Views

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01:49 min

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May 26, 2023

DOI:

10.3791/200225-v

DOI:

10.3791/200225-v

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01:49 min

May 26, 2023

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2 Views

Matteo Vitale*¹, Jiangtao Liang*², Igor Sharakhov, Federica Bernardini

¹Department of Life Sciences, Sir Alexander Fleming Building,Imperial College London, ²Department of Entomology,Virginia Tech

Tags

DNA Probe Genomic DNA PCR Reaction 18S RDNA Satellite AGY 53B Salmon Sperm DNA Ethanol Precipitation Sodium Acetate Hybridization Buffer Dextran Sulfate

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