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Chapters
- 00:00Title
- 00:57Introduction
- 01:24Preparation of Drosophila melanogaster Neuronal Muscular Junction (NMJ)
- 02:05Stimulation and FM Staining
- 02:43Fixing Neuromuscular Junction Preparations
- 03:19Photoconversion of FM1-43
- 04:49Processing the Sample for Electron Microscopy
- 07:29Representative Results
- 08:19Conclusion
FM dyes have been of invaluable help in the understanding of synaptic dynamics. FMs are normally followed under the fluorescent microscope during different stimulation conditions. However, photoconversion of FM dyes combined with electron microscopy allows the visualization of distinct synaptic vesicle pools, among other ultrastructure components, in synaptic boutons.
