A Phage Therapy against Pseudomonas aeruginosa Infection in Zebrafish Embryos
Published: May 31, 2024
Abstract
Source: Cafora, M., et al., A. Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos. J. Vis. Exp. (2020)
The video demonstrates the phage therapy technique targeting Pseudomonas aeruginosa infection in cystic fibrosis zebrafish embryos. Phage therapy effectively diminishes bacterial load and mitigates proinflammatory immune responses in CF zebrafish embryos infected with bacteria, leading to reduced CF embryo lethality.
Protocol
1. Microinjection of zebrafish embryos with bacteria and phage cocktail NOTE: To perform a systemic infection, the embryo must have blood circulation that usually starts after 26 hpf. After 26 hpf, dechorionate embryos with pronase solution prepared by dissolving pronase powder in E3 medium at a concentration of 2 mg/mL. Gently pipette the embryos to break the chorion. Discard the pronase/E3 medium and rinse the dish several times with fresh E3 to …
Disclosures
The authors have nothing to disclose.
Materials
| Bacto Agar | BD | 214010 | |
| Dulbecco's phospate buffered saline PBS | Sigma-Aldrich | D8537 | |
| Ethyl 3-aminobenzoate methanesulfonate | Sigma-Aldrich | 886-86-2 | Common name tricaine |
| Femtojet Micromanipulator | Eppendorf | 5247 | |
| Microinjection needles | Harvard apparatus | ||
| N-Phenylthiourea >=98% | Aldrich-P7629 | 103-85-5 | |
| Pronase | Sigma-Aldrich | 9036-06-0 | |
| Z-MOLDS Microinjection | Word Precision Instruments |
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Cite This Article
A Phage Therapy against Pseudomonas aeruginosa Infection in Zebrafish Embryos. J. Vis. Exp. (Pending Publication), e22264, doi: (2024).