An Ultrasound-Assisted Delivery of Antibodies in a Mouse Model
Published: March 29, 2024
Abstract
Source: Leinenga, G. et al., Delivery of Antibodies into the Brain Using Focused Scanning Ultrasound. J. Vis. Exp. (2020)
This video demonstrates a procedure of ultrasound-assisted antibody delivery in a mouse model. Retro-orbitally injected microbubbles oscillate under ultrasound, creating a transient disruption in the blood-brain barrier, permitting labeled antibodies to enter the brain.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. In-house microbubble preparation Weigh out a 9:1 molar ratio of 1,2-distearoyl-sn-glycero-3-phosphocholine and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethyleneglycol)-2000] (ammonium salt). 0.5 mg of lipid mixture is required per 1 mL of microbubble solution. Alternatively, lipids can be bought alre…
Disclosures
The authors have nothing to disclose.
Materials
| 1,2-distearoyl-sn-glycero-3-phosphocholine | Avanti | 850365C | |
| 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethyleneglycol)-2000] | Avanti | 880128C | |
| AlexaFluor 647 antibody labeling kit | Thermo Fisher | A20186 | |
| Chloroform | Sigma-Aldrich | 372978 | |
| Coulter Counter (Multisizer 4e) | |||
| Glycerol | Sigma-Aldrich | G5516 | |
| Head holder (model SG-4N, Narishige Japan) | |||
| Isoflow flow solution | Beckman Coulter | B43905 | |
| Octafluoropropane | Arcadophta | 0229NC | |
| Propylene Glycol | Sigma-Aldrich | P4347 | |
| TIPS (Therapy Imaging Probe System) | Philips Research | TIPS_007 | |
| Bitplane |
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Cite This Article
An Ultrasound-Assisted Delivery of Antibodies in a Mouse Model. J. Vis. Exp. (Pending Publication), e22094, doi: (2024).