An Assay to Detect Serum Anti-aquaporin-4 Immunoglobulin G
Published: March 29, 2024
Abstract
Source: Liu, C. et al., Human Serum Anti-aquaporin-4 Immunoglobulin G Detection by Cell-based Assay. J. Vis. Exp. (2019)
The video demonstrates a cell-based assay for detecting anti-aquaporin-4 immunoglobulin G using a biochip containing untransfected cells and transfected cells expressing aquaporin-4. This method has applications in diagnosing neuromyelitis optica spectrum disorders, confirmed by strong green fluorescence on the biochip.
Protocol
All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board. 1. Patient Enrollment and Blood Sample Collection Apply the laboratory detection of serum anti-aquaporin-4 immunoglobulin G (anti-AQP4 IgG) to clinic patients with the chief complaints and symptoms listed below. Perform physical examinations as w…
Representative Results
Figure 1: Flow chart of anti-AQP4 IgG detection protocol. AQP4-M1-transfected or untransfected EU 90 cells are fixed on the biochips. When adding serum to biochips, anti-AQP4 IgG in serum is captured by the fixed transfected cells. Then, fluorescein-labelled secondary antibody is applied to detect anti-AQP4 IgG. The fluorescence can be visualized by microscopy with various magnifications.</p…
Disclosures
The authors have nothing to disclose.
Materials
| Anti-aquaporin-4 IIFT | Euroimmun | FA 1128-2005-50 | Contains biochip slides coated with AQP4-M1 transfected and untransfected EU 90 cells, fluorescein-labelled anti-human IgG, anti-AQP4 antibody as positive control, antibody negative sample, salt for PBS pH 7.2, Tween 20 and embedding medium. |
| CellSens Dimension | OLYMPUS | N/A | photograph software |
| Gel & clot activator tube | Improve medical | 623040202 | From a local Chinese company |
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Cite This Article
An Assay to Detect Serum Anti-aquaporin-4 Immunoglobulin G. J. Vis. Exp. (Pending Publication), e22084, doi: (2024).