Induction of Necrotizing Enterocolitis in Human Epithelial Enteroid Model
Published: November 30, 2023
Abstract
Source: Ares, G. J., et al. A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis. J. Vis. Exp.(2019).
This video demonstrates the development of necrotizing enterocolitis disease in a human-derived neonatal enteroid model induced by lipopolysaccharides treatment. This model helps to study intestinal pathophysiology.
Protocol
All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board. 1. Reagent Preparation Prepare Culture Media stock solution for whole tissue collection: 1 L of Dulbecco's Modified Eagle Medium (DMEM), 110 mL of Fetal Bovine Serum (FBS), 11 mL of Penicillin-Streptomycin (final concentration 1%), and 1.1 mL…
Disclosures
The authors have nothing to disclose.
Materials
| 4% Paraformaldehyde | ThermoFisher | AAJ19943K2 | |
| Basement Membrane Matrix (Matrigel) | Corning | CB-40230C | |
| DMEM/F-12 | ThermoFisher | MT-16-405-CV | |
| Dulbecco's Modified Eagle Medium (DMEM) | ThermoFisher | 11-965-118 | |
| Dulbecco's Phosphate-Buffered Saline (DPBS) | ThermoFisher | 14190-144 | |
| Epidermal Growth Factor (EGF) | Sigma | E9644-.2MG | |
| Ethylenediaminetetraacetic acid (EDTA) | Sigma | EDS-500G | |
| Fetal Bovine Serum (FBS) | Gemini Bio-Pro | 100-125 | |
| Phosphate Buffered Saline (PBS) | Sigma | P5368-5X10PAK | |
| RPMI 1640 Medium | Invitrogen | 11875093 | |
| Tissue Processing Gel (Histogel) | ThermoFisher | 22-110-678 | |
| Lipopolysaccharide (LPS) | Sigma | L2630-25MG | |
| Gentamicin | Sigma | G5013-1G |
Cite This Article
Induction of Necrotizing Enterocolitis in Human Epithelial Enteroid Model. J. Vis. Exp. (Pending Publication), e21803, doi: (2023).