Fluorescent Orthotopic Mouse Model: Implanting GFP Expressing Cancer Cells into Mouse for Assessing Tumor Progression In Vivo
Published: April 30, 2023
Abstract
Source: Moreno, J. A., et al. Fluorescent orthotopic mouse model of pancreatic cancer. J. Vis. Exp.(2016)
This video demonstrates the protocol for implanting green fluorescent protein-expressing pancreatic cancer cells orthotopically into the pancreas of the mouse. The orthotopic mouse model helps in investigating the efficacy of novel therapeutics on primary and metastatic tumors.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the jove veterinary review board. 1. Surgical Implantation NOTE: Ensure that all surgical materials and instruments are sterile. Practice aseptic techniques at all times. Place a heating pad on the table and cover with a sterile drape. Set up the anesthesia machine and ensure all the supplies are within arm'…
Disclosures
The authors have nothing to disclose.
Materials
| Alcare or Foamed Antiseptic Handrub | Steris | 639680 | |
| TB Syringe 27 G 1/2 | Becton Dickinson | 305620 | |
| Isoflurane | Blutler Schein | 50562 | |
| Ketoprofen | Fort Dodge Animal Health | ||
| Surgical Scissors, 5.5" straight mayo | Henry Schein | 22-1600 | |
| PANC-1 GFP cell line | Anticancer, Inc | ||
| Small Animal Imaging System: iBox Scientia | UVP, LLC. Upland, CA. | Small Animal Imaging System to observe the fluorescent tumor in live animals | |
| SutureVet PGA 6-0 PGA | Henry Schein | 39010 | |
| PANC-1 GFP cell line | Anticancer, Inc | ||
| DPBS (Dubelcco's PhosphateBuffered saline) | Thermo Ficher Sci | 21300025 |
Cite This Article
Fluorescent Orthotopic Mouse Model: Implanting GFP Expressing Cancer Cells into Mouse for Assessing Tumor Progression In Vivo. J. Vis. Exp. (Pending Publication), e20318, doi: (2023).