Spatial Measurement of Tumor Interstitial Fluid Pressure: A Method to Measure the Interstitial Fluid Pressure
Published: April 30, 2023
Abstract
Source: Stapleton et. al., Measurements of Perfusion, Interstitial Fluid Pressure and Liposomes Accumulation in Solid Tumors. J. Vis. Exp. (2016).
This video describes a technique of measuring the interstitial fluid pressure within a tumor by perfusion imaging using an image-guided robotic system. Measurements are compared to the intra-tumoral accumulation of liposomes, within a solid tumor.
Protocol
1. Spatial Measurements of Tumor Interstitial Fluid Pressure To measure IFP connect the 25 G spinal needle to the pressure transducer and to the IFP acquisition system through 50 cm of PE20 polyethylene tubing. Flush the entire system with a heparin sulfate/saline solution (1:10). Sterilize the needle with 70% isopropyl before use. Turn on the acquisition system and launch the IFP acquisition software and load the settings files to calibrate the system to acquire IFP measurements in mmHg. Click…
Disclosures
The authors have nothing to disclose.
Materials
| Omnipaque (Iohexol) 300 mg of iodine/ml | GE Healthcare, CA | ||
| eXplore Locus Ultra micro-CT system | GE Healthcare, CA | Manipulated using CT-Console Software | |
| 27 G Catheter SURFLO Winged Infusion Set | Terumo Medical Products, USA | SV*27EL | |
| PE20 polyethylyne tubing | Becton Dickinson, USA | 427406 | |
| Pen tip 25 G × 3.5′′ Whitacre spinal needle | Becton Dickinson, USA | 405140 | IFP needle |
| P23XL pressure transducer | Harvard Apparatus, CA | P23XL | |
| PowerLab 4/35, Bridge Amp, with LabChart Pro 7.0 | ADInstruments Pty Ltd., USA | PL3504, FE221 | IFP acquisition system and acquisition software |
| CT-Sabre Small Animall Intervention system (CT-IFP Robot) | Parallax Innovations, CA | Manipulated using CT-IFP robot Control Software | |
| CT-IFP robot alignment software Custom Matlab software | |||
| DCE-CT Analysis Software | Custom Matlab software |
Cite This Article
Spatial Measurement of Tumor Interstitial Fluid Pressure: A Method to Measure the Interstitial Fluid Pressure. J. Vis. Exp. (Pending Publication), e20223, doi: (2023).