Subcutaneous Dihydrotestosterone (DHT) Treatment: Mouse Model of Polycystic Ovary Syndrome (PCOS)
Published: April 30, 2023
Abstract
Source: Xue, P., et al. A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome. J. Vis. Exp. (2018).
This article describes a way to prepare and insert dihydrotestosterone (DHT) pellets to model Polycystic Ovary Syndrome (PCOS) in mice. The example protocol demonstrates the procedure in mice used for reproductive and metabolic studies.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Create PCOS-like Mouse Model NOTE: The mice used in this study were a mixed background (C57/B6, CD1, 129Sv) and were maintained with food and water ad libitum in a 14/10 h light/dark cycle at 24 °C. DHT Pellet Preparation A…
Disclosures
The authors have nothing to disclose.
Materials
| Crystalline 5α-DHT powder | Sigma-Aldrich | A8380-1G | |
| Dow Corning Silastic tubing | Fisher Scientific | 11-189-15D | 0.04in/1mm inner diameter x 0.085in/2.15mm outer diameter |
| Medical adhesive silicone | Factor II, InC. | A-100 | |
| Goggles, lab coats, gloves and masks | |||
| 3 mL Syring | Becton, Dickinson and Company (BD) | 30985 | |
| Attached needle: 20G | BD | 305176 | |
| Ruler: any length than 10 cm with milimeter scale. | |||
| Xylazine | Vet one AnnSeA LA, MWI, Boise | NDC13985-704-10 | 100 mg/mL |
| Ketamine Hydrochloride | Hospira, Inc | NDC 0409-2051-05 | 100 mg/mL |
| Surgical staple | AutoClip® System, Fine Science Tool | 12020-00 | |
| Insulin syringe | BD | 329461 | 1/2 CC, low dose U-100 insulin syringe |
| Trocar | Innovative Research of America | MP-182 | |
| Razor blade | Fisher Scientific | 12-640 | |
| Clidox | Fisher Scientific | NC0089321 | |
| Surgical underpad | Fisher Scientific | 50587953 | Andwin Scientific 56616018 |
| Betadine Antiseptic Solution | Walgreens | ||
| 3M Vetbond (n-butyl cyanoacrylate) | 3M Science. Applied to Life |
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Cite This Article
Subcutaneous Dihydrotestosterone (DHT) Treatment: Mouse Model of Polycystic Ovary Syndrome (PCOS). J. Vis. Exp. (Pending Publication), e20014, doi: (2023).