JOVE JOURNAL

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Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research

Vojtech Gabriel¹, Christopher Zdyrski¹, Dipak K. Sahoo², Kimberly Dao³, Agnes Bourgois-Mochel², Jamie Kopper², Xi-Lei Zeng⁴, Mary K. Estes⁴, Jonathan P. Mochel^1,3, Karin Allenspach^2,3

¹Department of Biomedical Sciences, College of Veterinary Medicine,Iowa State University, ²Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University, ³3D Health Solutions Inc., ⁴Department of Molecular Virology and Microbiology,Baylor College of Medicine

Journal (Medicine)

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay

Tiago L. Duarte*^1,2, João V. Neves*^1,3,4

¹i3S - Instituto de Investigação e Inovação em Saúde,Universidade do Porto, ²Basic and Clinical Research on Iron Biology, IBMC - Instituto de Biologia Molecular e Celular,Universidade do Porto, ³Iron and Innate Immunity, IBMC - Instituto de Biologia Molecular e Celular,Universidade do Porto, ⁴ICBAS - Instituto de Ciências Biomédicas Abel Salazar,Universidade do Porto

Journal (Medicine)

Surgical Techniques to Optimize Ovarian Reserve during Laparoscopic Cystectomy for Ovarian Endometrioma

Kathryn Saturnino^1,2,3, Osaro Obanor^1,2,3, Cynthia Arvizo⁴, Julian A. Gingold^2,3,5

¹Department of Obstetrics & Gynecology and Women's Health,Montefiore Medical Center, ²Albert Einstein College of Medicine, ³Department of OB/GYN, 1400 Pelham Pkwy S,Jacobi Medical Center, ⁴Division of Minimally Invasive Gynecologic Surgery, Department of OB/GYN,Jacobi Medical Center, ⁵Division of Reproductive Endocrinology and Infertility, Department of Obstetrics & Gynecology and Women's Health,Montefiore Medical Center

Journal (Medicine)

Four-Dimensional Computed Tomography-Guided Valve Sizing for Transcatheter Pulmonary Valve Replacement

Xiaolin Sun*^1,2, Yimeng Hao*^1,2, Jonathan Frederik Sebastian Kiekenap¹, Jasper Emeis¹, Marvin Steitz², Alexander Breitenstein-Attach², Felix Berger^1,2, Boris Schmitt^1,2,3

¹Department of Pediatric Cardiology and Congenital Heart Disease,Charité University Medicine Berlin, ²Department of Pediatric Cardiology and Congenital Heart Disease,Deutsches Herzzentrum Berlin, ³DZHK (German Centre for Cardiovascular Research) and BMBF (German Ministry of Education and Research)

Journal (Medicine)

Endoscopic Cholesteatoma Surgery

Sven Beckmann¹, Georgios Mantokoudis¹, Stefan Weder¹, Urs Borner¹, Marco Caversaccio¹, Lukas Anschuetz¹

¹Department of Otorhinolaryngology, Head and Neck Surgery,Inselspital, Bern University Hospital, University of Bern

Journal (Medicine)

High-speed Video Microscopy Analysis for First-line Diagnosis of Primary Ciliary Dyskinesia

Rüdiger Schultz^1,2, Tiina Peromaa³, Heikki Lukkarinen², Varpu Elenius²

¹Allergy Centre,Tampere University Hospital, ²Department of Pediatrics,Turku University Hospital, ³Turku Bioscience Center,University of Turku and Åbo Akademi University

Journal (Medicine)

Induction of Myocardial Infarction and Myocardial Ischemia-Reperfusion Injury in Mice

Bingjie Lv*¹, Jin Zhou*¹, Shaolin He*¹, Yuqi Zheng¹, Wenlin Yang¹, Shangwei Liu¹, Chengxing Liu¹, Boyuan Wang¹, Dazhu Li¹, Jibin Lin¹

¹Department of Cardiology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology

Journal (Medicine)

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy

Xiaoting Mai*¹, Shaofen Huang*¹, Wen Chen¹, Tsz Kin Ng^1,2,3, Haoyu Chen¹

¹Joint Shantou International Eye Center,Shantou University and the Chinese University of Hong Kong, ²Shantou University Medical College, ³Department of Ophthalmology and Visual Sciences,the Chinese University of Hong Kong

Journal (Medicine)

Technique for Obtaining Mesenchymal Stem Cell from Adipose Tissue and Stromal Vascular Fraction Characterization in Long-Term Cryopreservation

Leniza Pola-Silva^1,2, Fabio Xerfan Nahas³, Flavia Nascimento², Tatiana Rabelo Santos², Andrea Moraes Malinverni¹, Adelson Alves², Lydia Masako Ferreira³, Maria Isabel Melaragno¹

¹Genetics Division,Universidade Federal de São Paulo (UNIFESP), ²TechLife Biotech,São Lucas Cell Therapy Group, ³Division of Plastic Surgery,Universidade Federal de São Paulo (UNIFESP)

Journal (Medicine)

An Epithelial Abrasion Model for Studying Corneal Wound Healing

Prince K. Akowuah¹, Angie De La Cruz¹, C. Wayne Smith², Rolando E. Rumbaut^2,3, Alan R. Burns^1,2

¹College of Optometry,University of Houston, ²Children’s Nutrition Research Center,Baylor College of Medicine, ³Center for Translational Research on Inflammatory Diseases (CTRID),Michael E. DeBakey Veterans Affairs Medical Center

Journal (Medicine)

Isolation of Adipose Derived Regenerative Cells for the Treatment of Erectile Dysfunction Following Radical Prostatectomy

Sabrina T. Hansen^1,2, Charlotte H. Jensen^2,3,4, Jens A. Sørensen^2,4,5, Søren P. Sheikh^2,3,4, Lars Lund^1,2,4

¹Department of Urology,Odense University Hospital, ²Clinical Institute,University of Southern Denmark, ³Laboratory of Molecular and Cellular Cardiology, Department of Clinical Biochemistry and Pharmacology,Odense University Hospital, ⁴Center for Vascular Regeneration,Odense University Hospital, ⁵Research Unit for Plastic Surgery,Odense University Hospital

Journal (Medicine)

Culture and Imaging of Human Nasal Epithelial Organoids

Zhongyu Liu^1,2, Justin D. Anderson^1,2, Jennifer Natt^1,2, Jennifer S. Guimbellot^1,2

¹Gregory Fleming James Cystic Fibrosis Research Center,University of Alabama at Birmingham (UAB), ²Department of Pediatrics, Division of Pulmonary and Sleep Medicine,UAB

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