JOVE JOURNAL

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An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings

Justen Manasa¹, Siva Danaviah¹, Sureshnee Pillay¹, Prevashinee Padayachee¹, Hloniphile Mthiyane¹, Charity Mkhize¹, Richard John Lessells¹, Christopher Seebregts², Tobias F. Rinke de Wit³, Johannes Viljoen¹, David Katzenstein⁴, Tulio De Oliveira¹

¹Africa Centre for Health and Population Studies, College of Health Sciences,University of KwaZulu-Natal, Durban, South Africa, ²Unit D11,Jembi Health Systems, ³Academic Medical Center, Department of Global Health, Amsterdam Institute for Global Health and Development (AIGHD),University of Amsterdam, ⁴Division of Infectious Diseases and Geographic Medicine, Centre for AIDS Research,Stanford Medical School

Journal (Medicine)

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

Melanie R. Rutkowski*¹, Michael J. Allegrezza*¹, Nikolaos Svoronos¹, Amelia J. Tesone¹, Tom L. Stephen¹, Alfredo Perales-Puchalt¹, Jenny Nguyen¹, Paul J. Zhang², Steven N. Fiering³, Julia Tchou^4,5,6, Jose R. Conejo-Garcia¹

¹Tumor Microenvironment and Metastasis Program,Wistar Institute, ²Department of Pathology and Lab Medicine, Perelman School of Medicine,University of Pennsylvania, ³Department of Microbiology and Immunology and Department of Genetics,Geisel School of Medicine at Dartmouth, ⁴Division of Endocrine and Oncologic Surgery, Department of Surgery, Perelman School of Medicine,University of Pennsylvania, ⁵Rena Rowan Breast Center, Abramson Cancer Center,University of Pennsylvania, ⁶Center for Advanced Medicine,University of Pennsylvania

Journal (Medicine)

Bronchoalveolar Lavage (BAL) for Research; Obtaining Adequate Sample Yield

Andrea M. Collins^1,2, Jamie Rylance³, Daniel G. Wootton⁴, Angela D. Wright^3,5, Adam K. A. Wright^1,3, Duncan G. Fullerton^3,6, Stephen B. Gordon³

¹Biomedical Research Centre in Microbial Diseases,National Institute for Health Research, ²Respiratory Infection Group,Royal Liverpool and Broadgreen University Hospital Trust, ³Respiratory Infection Group,Liverpool School of Tropical Medicine, ⁴Institute of Infection and Global Health,University of Liverpool, ⁵Comprehensive Local Research Network,Royal Liverpool and Broadgreen University Hospital Trust, ⁶Department of Respiratory Research,University Hospital Aintree

Journal (Medicine)

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro

Daniela Meier*¹, Carmen Campanile*¹, Sander M. Botter¹, Walter Born¹, Bruno Fuchs¹

¹Laboratory for Orthopedic Research,Balgrist University Hospital, Zurich, Switzerland

Journal (Medicine)

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Samuel J. Vidal¹, S. Aidan Quinn¹, Janis de la Iglesia-Vicente¹, Dennis M. Bonal¹, Veronica Rodriguez-Bravo², Adolfo Firpo-Betancourt¹, Carlos Cordon-Cardo¹, Josep Domingo-Domenech¹

¹Department of Pathology,Icahn School of Medicine at Mount Sinai, ²Molecular Biology Program,Memorial Sloan-Kettering Cancer Center

Journal (Medicine)

A Mouse Tumor Model of Surgical Stress to Explore the Mechanisms of Postoperative Immunosuppression and Evaluate Novel Perioperative Immunotherapies

Lee-Hwa Tai¹, Christiano Tanese de Souza¹, Shalini Sahi¹, Jiqing Zhang^1,2,4, Almohanad A Alkayyal^1,3,5, Abhirami Anu Ananth^1,3, Rebecca A.C. Auer^1,6

¹Centre for Innovative Cancer Research,Ottawa Hospital Research Institute, ²Department of Cellular and Molecular Medicine,University of Ottawa, ³Department of Biochemistry, Microbiology and Immunology,University of Ottawa, ⁴Department of Neurosurgery,The Second Hospital of Shandong University, ⁵Department of Medical Laboratory Technology,University of Tabuk, ⁶Department of Surgery,Ottawa General Hospital

Journal (Medicine)

RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma

Hongwei Wang¹, Mindy Xiao-Ming Wang¹, Nan Su¹, Li-chong Wang¹, Xingyong Wu¹, Son Bui¹, Allissa Nielsen¹, Hong-Thuy Vo¹, Nina Nguyen¹, Yuling Luo¹, Xiao-Jun Ma¹

¹Advanced Cell Diagnostics, Inc.

Journal (Medicine)

A Three-dimensional Tissue Culture Model to Study Primary Human Bone Marrow and its Malignancies

Mukti R. Parikh¹, Andrew R. Belch^2,3, Linda M Pilarski^2,3, Julia Kirshner¹

¹Department of Biological Sciences,Purdue University, ²Department of Oncology,University of Alberta, ³Cross Cancer Institute

Journal (Medicine)

Ultrasound Imaging-guided Intracardiac Injection to Develop a Mouse Model of Breast Cancer Brain Metastases Followed by Longitudinal MRI

Heling Zhou¹, Dawen Zhao¹

¹Radiology,University of Texas Southwestern Medical Center

Journal (Medicine)

Utility of Dissociated Intrinsic Hand Muscle Atrophy in the Diagnosis of Amyotrophic Lateral Sclerosis

Parvathi Menon^1,2, Steve Vucic^1,2

¹Department of Neurology,Westmead Hospital, ²Sydney Medical School,University of Sydney, Australia

Journal (Medicine)

Reproducable Paraplegia by Thoracic Aortic Occlusion in a Murine Model of Spinal Cord Ischemia-reperfusion

Marshall T. Bell¹, T. Brett Reece¹, Phillip D. Smith¹, Joshua Mares¹, Michael J. Weyant¹, Joseph C. Cleveland Jr.¹, Kirsten A. Freeman¹, David A. Fullerton¹, Ferenc Puskas²

¹Department of Surgery, Division of Cardiothoracic Surgery,University of Colorado, ²Department of Anesthesiology,University of Colorado

Journal (Medicine)

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications

Lori E. Lowes^1,2, Benjamin D. Hedley³, Michael Keeney^3,4, Alison L. Allan^1,2,4,5

¹London Regional Cancer Program,London Health Sciences Centre, ²Department of Anatomy & Cell Biology, Schulich School of Medicine and Dentistry,Western University, ³Special Hematology/Flow Cytometry,London Health Sciences Centre, ⁴Lawson Health Research Institute, ⁵Department of Oncology,Western University

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