JOVE JOURNAL

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Optimized Protocols for Mycobacterium leprae Strain Management: Frozen Stock Preservation and Maintenance in Athymic Nude Mice

Ana Paula Fávaro Trombone¹, Sílvia Cristina Barbosa Pedrini², Suzana Madeira Diório³, Andréa de Faria Fernandes Belone¹, Luciana Raquel Vicenzi Fachin¹, Dejair Caitano do Nascimento⁴, Patricia Sammarco Rosa²

¹Department of Pathology,Instituto Lauro de Souza Lima (ILSL), ²Laboratory Animal House,Instituto Lauro de Souza Lima (ILSL), ³Department of Microbiology,Instituto Lauro de Souza Lima (ILSL), ⁴Department of Pharmacology,Instituto Lauro de Souza Lima (ILSL)

Journal (Immunology and Infection)

A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress

Shishir Shetty¹, Christopher J. Weston¹, David H. Adams¹, Patricia F. Lalor¹

¹NIHR Biomedical Research Unit, Centre for Liver Research, School of Immunity and Infection,University of Birmingham

Journal (Immunology and Infection)

Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen

Kwame Twumasi-Boateng¹, Maureen Berg¹, Michael Shapira¹

¹Department of Integrative Biology,University of California, Berkeley

Journal (Immunology and Infection)

Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice

Marcella Facchini^1,2, Ida De Fino^1,2, Camilla Riva^1,2, Alessandra Bragonzi¹

¹Infections and Cystic Fibrosis Unit, Division of Immunology, Transplantation and Infectious Diseases,San Raffaele Scientific Institute, ²Italian Cystic Fibrosis Research Foundation

Journal (Immunology and Infection)

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors

Haiyan S. Li¹, Stephanie S. Watowich^1,2

¹Department of Immunology,The University of Texas MD Anderson Cancer Center, ²The University of Texas Graduate School of Biomedical Sciences

Journal (Immunology and Infection)

Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians

Laura A. Brannelly¹

¹School of Public Health, Tropical Medicine and Rehabilitation Sciences,James Cook University

Journal (Immunology and Infection)

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

T. Ryan Withers¹, Yeshi Yin¹, Hongwei D. Yu¹

¹Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine,Marshall University

Journal (Immunology and Infection)

Study of Phagolysosome Biogenesis in Live Macrophages

Marc Bronietzki¹, Bahram Kasmapour¹, Maximiliano Gabriel Gutierrez^1,2

¹Research Group Phagosome Biology,Helmholtz Centre for Infection Research, ²Division of Mycobacterial Research,National Institute for Medical Research

Journal (Immunology and Infection)

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Stephan Uphoff^1,2, David J. Sherratt¹, Achillefs N. Kapanidis²

¹Microbiology Unit, Department of Biochemistry,University of Oxford, ²Biological Physics Research Group, Clarendon Laboratory, Department of Physics,University of Oxford

Journal (Immunology and Infection)

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Junjie Zhang¹, Lining Zhu¹, Pinghui Feng¹

¹Department of Microbiology and Immunology,Keck School of Medicine, University of Southern California

Journal (Immunology and Infection)

Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction

Maha Habib¹, Barbara D. Porter¹, Catherine Satzke^1,2

¹Pneumococcal Research,Murdoch Childrens Research Institute, ²Department of Microbiology & Immunology,The University of Melbourne

Journal (Immunology and Infection)

Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Adi Kliot^1,2, Svetlana Kontsedalov¹, Galina Lebedev¹, Marina Brumin¹, Pakkianathan Britto Cathrin¹, Julio Massaharu Marubayashi¹, Marisa Skaljac^1,3, Eduard Belausov⁴, Henryk Czosnek², Murad Ghanim¹

¹Department of Entomology,Volcani Center, ²Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem, ³Department of Applied Sciences,Institute for Adriatic Crops and Karst Reclamation, ⁴The Institute of Plant Sciences,Volcani Center

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