JOVE JOURNAL

면역학 및 감염병학

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Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

Meisam Naeimi Kararoudi¹, Hamid Dolatshad², Prashant Trikha¹, Syed-Rehan A. Hussain³, Ezgi Elmas¹, Jennifer A. Foltz¹, Jena E. Moseman¹, Aarohi Thakkar¹, Robin J. Nakkula¹, Margaret Lamb¹, Nitin Chakravarti¹, K. John McLaughlin³, Dean A. Lee¹

¹Center for Childhood Cancer and Blood Disease,Nationwide Children's Hospital, ²Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine,University of Oxford, ³Center for Clinical and Translational Research,Nationwide Children's Hospital Research Institute

Journal (Immunology and Infection)

Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae

Helen Weavers^1,2, Anna Franz¹, Will Wood³, Paul Martin^1,4

¹School of Biochemistry, Biomedical Sciences,University of Bristol, ²School of Cellular and Molecular Medicine, Biomedical Sciences,University of Bristol, ³MRC Centre for Inflammation Research,University of Edinburgh, Queens Medical Research Institute, ⁴School of Physiology, Pharmacology, and Neuroscience, Biomedical Sciences,University of Bristol

Journal (Immunology and Infection)

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Alba Llibre*^1,2, Vincent Bondet*^1,2, Mathieu P. Rodero³, David Hunt⁴, Yanick J. Crow^3,5, Darragh Duffy^1,2

¹Immunobiology of Dendritic Cells,Institut Pasteur, ²INSERM U1223, ³Laboratory of Neurogenetics and Neuroinflammation, INSERM UMR1163,Institut Imagine, ⁴MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine,University of Edinburgh, ⁵Manchester Centre for Genomic Medicine,University of Manchester

Journal (Immunology and Infection)

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

Rouven Schoppmeyer¹, Renping Zhao¹, Markus Hoth¹, Bin Qu¹

¹Department of Biophysics, Center for Integrative Physiology and Molecular Medicine (CIPMM), School of Medicine,Saarland University

Journal (Immunology and Infection)

Deep Dermal Injection As a Model of Candida albicans Skin Infection for Histological Analyses

William Santus¹, Francesca Mingozzi¹, Marina Vai¹, Francesca Granucci*¹, Ivan Zanoni*^1,2

¹Department of Biotechnology and Biosciences,University of Milano-Bicocca, ²Harvard Medical School and Division of Gastroenterology,Boston Children's Hospital

Journal (Immunology and Infection)

Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics

Hyunryul Ryu¹, Kyungyong Choi^1,2, Yanyan Qu³, Taehong Kwon^1,2, Janet S. Lee^3,5, Jongyoon Han^1,2,4

¹Research Laboratory of Electronics,Massachusetts Institute of Technology, ²Department of Electrical Engineering and Computer Science,Massachusetts Institute of Technology, ³Department of Medicine,University of Pittsburgh, ⁴Department of Biological Engineering,Massachusetts Institute of Technology, ⁵Vascular Medicine Institute,University of Pittsburgh

Journal (Immunology and Infection)

Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues

Remco J. Molenaar¹, Mohammed Khurshed¹, Vashendriya V.V. Hira¹, Cornelis J.F. Van Noorden¹

¹Cancer Center Amsterdam, Department of Medical Biology, Academic Medical Center,University of Amsterdam, Amsterdam, The Netherlands

Journal (Immunology and Infection)

Imaging Ca²⁺ Responses During Shigella Infection of Epithelial Cells

Yasmine Smail^1,2,3,4, Chunhui Sun^1,2,3,4, Laurent Combettes⁵, Guy Tran Van Nhieu^1,2,3,4

¹Equipe Communication Intercellulaire et Infections Microbiennes, Centre de Recherche Interdisciplinaire en Biologie (CIRB),Collège de France, ²Institut National de la Santé et de la Recherche Médicale (Inserm), U1050, ³Centre Nationale de la Recherche Scientifique (CNRS), UMR7241, ⁴MEMOLIFE Laboratory of Excellence and Paris Sciences et Lettres, ⁵Inserm, UMRS1174,Université Paris Sud

Journal (Immunology and Infection)

Extraction of Hemocytes from Drosophila melanogaster Larvae for Microbial Infection and Analysis

Aoi Hiroyasu¹, David C. DeWitt², Alan G. Goodman¹

¹School of Molecular Biosciences, College of Veterinary Medicine,Washington State University, ²Integrative Physiology and Neuroscience, College of Veterinary Medicine,Washington State University

Journal (Immunology and Infection)

Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

Juntao Liu*¹, Lihui Zhu*¹, Lihui Wang^1,2, Yongjun Chen¹, Bikash Ranjan Giri¹, Jianjun Li², Guofeng Cheng¹

¹Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology,Ministry of Agriculture, ²Tianjin Agricultural University

Journal (Immunology and Infection)

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Andreas B. den Hartigh¹, Susan L. Fink¹

¹Department of Laboratory Medicine,University of Washington

Journal (Immunology and Infection)

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Krista M. Armbruster¹, Timothy C. Meredith¹

¹Department of Biochemistry and Molecular Biology,Pennsylvania State University

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