JOVE JOURNAL

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Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

Marcus Gereke^1,2, Andrea Autengruber^1,2, Lothar Gröbe³, Andreas Jeron², Dunja Bruder^1,2, Sabine Stegemann-Koniszewski¹

¹Research Group Immune Regulation,Helmholtz Centre for Infection Research, ²Research Group Infection Immunology, Institute of Medical Microbiology,Otto-von-Guericke University , ³Department of Experimental Immunology,Helmholtz Centre for Infection Research

Journal (Immunology and Infection)

Recurrent Herpetic Stromal Keratitis in Mice, a Model for Studying Human HSK

Jessica Morris¹, Patrick M. Stuart¹, Megan Rogge¹, Chloe Potter¹, Nipun Gupta¹, Xiao-Tang Yin¹

¹Department of Ophthalmology,Saint Louis University

Journal (Immunology and Infection)

Using the BLT Humanized Mouse as a Stem Cell based Gene Therapy Tumor Model

Dimitrios N. Vatakis^1,2,3, Gregory C. Bristol^1,2, Sohn G. Kim^1,2, Bernard Levin^1,2, Wei Liu⁴, Caius G. Radu⁴, Scott G. Kitchen^1,2,3, Jerome A. Zack^1,2,5

¹Department of Medicine, Division of Hematology-Oncology,David Geffen School of Medicine at UCLA, ²UCLA AIDS Institute, ³Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA, ⁴Department of Medical and Molecular Pharmacology,David Geffen School of Medicine at UCLA, ⁵Department of Microbiology, Immunology and Molecular Genetics,David Geffen School of Medicine at UCLA

Journal (Immunology and Infection)

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis

Nalini Ramarao¹, Christina Nielsen-Leroux¹, Didier Lereclus¹

¹INRA, Micalis UMR1319, France

Journal (Immunology and Infection)

Rearing and Injection of Manduca sexta Larvae to Assess Bacterial Virulence

Elizabeth Hussa¹, Heidi Goodrich-Blair¹

¹Department of Bacteriology,University of Wisconsin-Madison

Journal (Immunology and Infection)

Quantitative Measurement of the Immune Response and Sleep in Drosophila

Tzu-Hsing Kuo¹, Arun Handa¹, Julie A. Williams¹

¹Center for Sleep and Circadian Neurobiology,University of Pennsylvania Perelman School of Medicine

Journal (Immunology and Infection)

A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay

Maciej T. Nogalski^1,2, Gary C.T. Chan³, Emily V. Stevenson^1,2, Donna K. Collins-McMillen^1,2, Andrew D. Yurochko^1,2,4

¹Department of Microbiology and Immunology,Louisiana State University Health Sciences Center, ²Center for Molecular and Tumor Virology,Louisiana State University Health Sciences Center, ³Department of Microbiology and Immunology,SUNY Upstate Medical University, ⁴Feist-Weiller Cancer Center,Louisiana State University Health Sciences Center

Journal (Immunology and Infection)

In Vivo Imaging Systems (IVIS) Detection of a Neuro-Invasive Encephalitic Virus

Allison Poussard*¹, Michael Patterson*¹, Katherine Taylor¹, Alexey Seregin¹, Jeanon Smith¹, Jennifer Smith¹, Milagros Salazar¹, Slobodan Paessler¹

¹Experimental Pathology,University of Texas Medical Branch

Journal (Immunology and Infection)

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Ryan D. Heselpoth¹, Daniel C. Nelson¹

¹Institute for Bioscience and Biotechnology Research,University of Maryland

Journal (Immunology and Infection)

Protein Misfolding Cyclic Amplification of Prions

Samuel E. Saunders¹, Jason C. Bartz², Ronald A. Shikiya²

¹Department of Civil Engineering,University of Nebraska at Lincoln, ²Department of Medical Microbiology and Immunology,Creighton University

Journal (Immunology and Infection)

piggyBac Transposon System Modification of Primary Human T Cells

Sunandan Saha^1,2, Yozo Nakazawa³, Leslie E. Huye^4,5, Joseph E. Doherty^2,6, Daniel L. Galvan², Cliona M. Rooney^4,5,7, Matthew H. Wilson^1,2,4,8

¹Program in Translational Biology and Molecular Medicine,Baylor College of Medicine , ²Department of Medicine, Division of Nephrology,Baylor College of Medicine , ³Department of Immunology and Pathology,Shinshu University School of Medicine, ⁴Center for Cell and Gene Therapy,Baylor College of Medicine , ⁵Department of Pediatrics,Baylor College of Medicine , ⁶Program in Cell and Molecular Biology,Baylor College of Medicine , ⁷Department of Molecular Virology and Microbiology,Baylor College of Medicine , ⁸Michael E. DeBakey VA Medical Center

Journal (Immunology and Infection)

Bioluminescent Bacterial Imaging In Vivo

Chwanrow K. Baban*¹, Michelle Cronin*¹, Ali R. Akin¹, Anne O'Brien¹, Xuefeng Gao¹, Sabin Tabirca¹, Kevin P. Francis¹, Mark Tangney¹

¹Cork Cancer Research Centre, BioSciences Institute,University College Cork

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