JOVE JOURNAL

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CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Linyuan Ma*¹, Lydia Jang*¹, Jian Chen², Jun Song¹, Dongshan Yang¹, Jifeng Zhang¹, Y. Eugene Chen¹, Jie Xu¹

¹Center for Advanced Models for Translational Sciences and Therapeutics, Department of Internal Medicine,University of Michigan Medical Center, ²Celetrix LLC

Journal (Genetics)

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Snorre Gulla¹, Saima Nasrin Mohammad¹, Duncan John Colquhoun^1,2

¹Fish Health Research Group,Norwegian Veterinary Institute, ²University of Bergen

Journal (Genetics)

Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

Sophie Outh-Gauer¹, Jérémy Augustin¹, Marion Mandavit², Ophélie Grard², Thomas Denize¹, Marine Nervo¹, Charles Lépine¹, Marc Rassy², Eric Tartour^2,3, Cécile Badoual^1,2

¹Department of Pathology, Georges Pompidou European Hospital, Assistance publique - Hôpitaux de Paris (APHP),Paris Descartes University, ²Paris Cardiovascular Research Center (PARCC), Institut national de la santé et de la recherche médicale (Inserm) U970, ³Laboratory of Immunology, Georges Pompidou European Hospital, APHP,Paris Descartes University

Journal (Genetics)

Generation of Tumor Organoids from Genetically Engineered Mouse Models of Prostate Cancer

Kristine M. Wadosky¹, Yanqing Wang¹, Xiaojing Zhang¹, David W. Goodrich¹

¹Department of Pharmacology & Therapeutics,Roswell Park Comprehensive Cancer Center

Journal (Genetics)

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior

John A. Schmidt¹, Janice E. Knepper¹

¹Department of Biology,Villanova University

Journal (Genetics)

An Assay for Quantifying Protein-RNA Binding in Bacteria

Noa Katz*¹, Roni Cohen*¹, Orna Atar¹, Sarah Goldberg¹, Roee Amit^1,2

¹Department of Biotechnology and Food Engineering,Technion-Israel Institute of Technology, ²Russell Berrie Nanotechnology Institute,Technion-Israel Institute of Technology

Journal (Genetics)

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Andreas Mueller¹, Kerstin Fischer², Roland Suluku³, Thomas Hoenen¹

¹Institute of Molecular Virology and Cell Biology,Friedrich-Loeffler-Institut, ²Institute of Novel and Emerging Infectious Diseases,Friedrich-Loeffler-Institut, ³Department of Animal Science,Njala University

Journal (Genetics)

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

Piotr Przanowski¹, Zeming Zheng¹, Urszula Wasko¹, Sanchita Bhatnagar^1,2

¹Department of Biochemistry and Molecular Genetics,University of Virginia School of Medicine, ²Department of Neuroscience,University of Virginia School of Medicine

Journal (Genetics)

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Hyun Ah Seo¹, Cho Yean Hwang¹, Sokviseth Moeng¹, Jong Kook Park¹

¹Department of Biomedical Science and Research Institute for Bioscience & Biotechnology,Hallym University

Journal (Genetics)

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Tiffany Kaul¹, Maria E. Morales¹, Emily Smither¹, Melody Baddoo^1,2, Victoria P. Belancio^1,3, Prescott Deininger^1,4

¹Tulane Cancer Center,Tulane University, ²Department of Pathology,Tulane University, ³Department of Structural and Cellular Biology,Tulane University, ⁴Department of Epidemiology,Tulane University

Journal (Genetics)

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

Zachary J. Heins^1,2, Christopher P. Mancuso^1,2, Szilvia Kiriakov^1,3, Brandon G. Wong^1,2, Caleb J. Bashor⁴, Ahmad S. Khalil^1,2,5

¹Biological Design Center,Boston University, ²Department of Biomedical Engineering,Boston University, ³Program in Molecular Biology, Cell Biology and Biochemistry,Boston University, ⁴Department of Bioengineering,Rice University, ⁵Wyss Institute for Biologically Inspired Engineering,Harvard University

Journal (Genetics)

Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

Siming Liu^1,2, Mikako Harata^1,2, Joseph A. Promes^1,2, Anthony J. Burand^2,3, James A. Ankrum^2,3, Yumi Imai^1,2

¹Department of Internal Medicine, Carver College of Medicine,University of Iowa, ²Fraternal Order of Eagles Diabetes Research Center,University of Iowa, ³Roy J. Carver Department of Biomedical Engineering,University of Iowa

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