JOVE JOURNAL

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Analysis of Non-Human Primate Pancreatic Islet Oxygen Consumption

Joseph M. Elsakr¹, Charles Deeter², Valerie Ricciardi³, Maureen Gannon^1,4,5,6

¹Department of Molecular Physiology and Biophysics,Vanderbilt University, ²Agilent Technologies, ³Department of Biological Sciences,Vanderbilt University, ⁴Department of Veterans Affairs Tennessee Valley, ⁵Department of Medicine,Vanderbilt University Medical Center, ⁶Department of Cell and Developmental Biology,Vanderbilt University

Journal (Developmental Biology)

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Meng Wu¹, Divya Kriti¹, Harm van Bakel^1,2, Ethylin Wang Jabs¹, Greg Holmes¹

¹Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, ²Icahn Institute for Data Science and Genomic Technology,Icahn School of Medicine at Mount Sinai

Journal (Developmental Biology)

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Hiromi Shimojo¹, Ryoichiro Kageyama^1,2,3,4

¹Institute for Frontier Life and Medical Sciences,Kyoto University, ²Institute for Integrated Cell-Material Sciences (iCeMS),Kyoto University, ³Kyoto University Graduate School of Medicine, ⁴Kyoto University Graduate School of Biostudies

Journal (Developmental Biology)

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function

Robert Mahen^1,2, Reiner Schulte³

¹Photonics Group, Department of Physics,Imperial College London, ²The Medical Research Council Cancer Unit, Hutchison/MRC Research Centre, Cambridge Biomedical Campus,University of Cambridge, ³Cambridge Institute for Medical Research, Cambridge Biomedical Campus,University of Cambridge

Journal (Developmental Biology)

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Daniela Avila-González¹, Guadalupe García-López¹, Néstor E. Díaz-Martínez², Héctor Flores-Herrera³, Anayansi Molina-Hernández¹, Wendy Portillo⁴, Néstor F. Díaz¹

¹Departamento de Fisiología y Desarrollo Celular,Instituto Nacional de Perinatología, ²Biotecnología Médica y Farmacéutica CONACYT,Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), ³Departamento de Inmunobioquímica,Instituto Nacional de Perinatología, ⁴Instituto de Neurobiología, UNAM

Journal (Developmental Biology)

In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

Christina Rou Hsu^1,2, Rain Xiong^1,2, Kenji Sugioka^1,2

¹Department of Zoology,The University of British Columbia, ²Life Sciences Institute,The University of British Columbia

Journal (Developmental Biology)

Quantification of Self-renewal in Murine Mammosphere Cultures

Thalia Vlachou¹, Xieraili Aobuli¹, Errico D'Elia¹, Angela Santoro¹, Maria Cristina Moroni¹, Pier Giuseppe Pelicci^1,2

¹Department of Experimental Oncology, IEO,European Institute of Oncology IRCCS, ²Department of Oncology and Hemato-Oncology,University of Milan

Journal (Developmental Biology)

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Tânia Martins Garcia¹, Marit Navis¹, Manon E. Wildenberg¹, Ruurd M. van Elburg², Vanesa Muncan¹

¹Department of Gastroenterology and Hepatology, Tytgat Institute for Intestinal and Liver Research, Amsterdam UMC, AG&M,University of Amsterdam, ²Department of Pediatrics, Amsterdam UMC,University of Amsterdam

Journal (Developmental Biology)

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Giorgia Scapin^1,2,3, Marie C. Goulard^1,2,3, Priyanka R. Dharampuriya^1,2,3, Jennifer L. Cillis^1,2,3, Dhvanit I. Shah^1,2,3

¹Nationwide Children's Hospital, ²The Ohio State University College of Medicine, ³The Ohio State University Comprehensive Cancer Center

Journal (Developmental Biology)

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Rita Silvério-Alves^1,2,3, Andreia M. Gomes³, Ilia Kurochkin⁴, Kateri A. Moore^5,6, Carlos-Filipe Pereira^1,2,3

¹Molecular Medicine and Gene Therapy, Lund Stem Cell Center,Lund University, ²Wallenberg Center for Molecular,Lund University, ³Center for Neuroscience and Cell Biology,University of Coimbra, ⁴Skolkovo Institute of Science and Technology, ⁵Department of Cell, Developmental and Regenerative Biology,Icahn School of Medicine at Mount Sinai, ⁶Black Family Stem Cell Institute,Icahn School of Medicine at Mount Sinai

Journal (Developmental Biology)

Imaging Dpp Release from a Drosophila Wing Disc

Laura Faith George¹, Emily Anne Bates¹

¹Department of Pediatrics,University of Colorado School of Medicine

Journal (Developmental Biology)

A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development

Renat N. Khaliullin^1,2,3, Jeffrey M. Hendel^1,2, Adina Gerson-Gurwitz^1,2, Shaohe Wang^1,4,5, Stacy D. Ochoa^1,6, Zhiling Zhao^1,7, Arshad Desai^1,2, Karen Oegema^1,2, Rebecca A. Green^1,2

¹Ludwig Institute for Cancer Research, San Diego, ²Department of Cellular and Molecular Medicine,University of California, San Diego, ³Recursion Pharmaceuticals, ⁴Biomedical Sciences Graduate Program,University of California, San Diego, ⁵Cell Biology Section, National Institute of Dental and Craniofacial Research,National Institutes of Health, ⁶Department of Biology,San Diego State University, ⁷Developmental and Stem Cell Biology Graduate Program,University of California, San Francisco

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