JOVE JOURNAL

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Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

Bridget T. Jacques-Fricke¹, Julaine Roffers-Agarwal^2,3, Callie M. Gustafson^2,3, Laura S. Gammill^2,3

¹Department of Biology and Neuroscience Program,Hamline University, ²Department of Genetics, Cell Biology and Development,University of Minnesota, ³Developmental Biology Center,University of Minnesota

Journal (Developmental Biology)

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

Yoko Tanimoto*¹, Natsuki Mikami*^1,2, Miyuki Ishida¹, Natsumi Iki¹, Kanako Kato¹, Fumihiro Sugiyama¹, Satoru Takahashi¹, Seiya Mizuno¹

¹Laboratory Animal Resource Center and Trans-Border Medical Research Center,University of Tsukuba, ²Ph.D. Program in Human Biology, School of Integrative and Global Majors,University of Tsukuba

Journal (Developmental Biology)

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development

Nishant Singh*^1,2, Anubhav Prakash*¹, Suraj Ranganath Chakravarthy*¹, Raman Kaushik*¹, Raj K. Ladher¹

¹National Centre for Biological Sciences,Tata Institute of Fundamental Research, ²The University of Trans-Disciplinary Health Sciences and Technology

Journal (Developmental Biology)

Fixation of Embryonic Mouse Tissue for Cytoneme Analysis

Eric T. Hall¹, Christina A. Daly^1,2, Yan Zhang¹, Miriam E. Dillard¹, Stacey K. Ogden¹

¹Department of Cell and Molecular Biology,St. Jude Children’s Research Hospital, ²St. Jude Graduate School of Biomedical Sciences

Journal (Developmental Biology)

Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity

Michael Attwaters*¹, Jeffrey J. Kelu*¹, Tapan G. Pipalia¹, Simon M. Hughes¹

¹Randall Centre for Cell and Molecular Biophysics, School of Basic and Medical Biosciences,King’s College London

Journal (Developmental Biology)

Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells

Andrew Berical^1,2, Mary Lou Beermann², Shingo Suzuki³, Jake LeSuer², Taylor Matte², Brian Davis³, Darrell Kotton^1,2, Finn Hawkins^1,2

¹Pulmonary Center,Boston University School of Medicine, ²Center for Regenerative Medicine,Boston University and Boston Medical Center, ³Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine,University of Texas Health Science Center

Journal (Developmental Biology)

Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing

Changchun Dong*¹, Bikash Lamichhane*¹, Yongxu Zhang¹, Xiaofang Wang¹

¹Department of Biomedical Sciences,Texas A&M School of Dentistry

Journal (Developmental Biology)

FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle

Giulia Riparini¹, James M. Simone², Vittorio Sartorelli¹

¹Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS),National Institutes of Health (NIH), ²Flow Cytometry Section, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS),National Institutes of Health (NIH)

Journal (Developmental Biology)

In Ovo Intravascular Injection in Chicken Embryos

Kai Jin^1,2, Jing Zhou^1,2, Gaoyuan Wu^1,2, Ziyi Lian^1,2, Zongyi Zhao^1,2, Shujian Zhou^1,2, Chen Chen^1,2, Hongyan Sun^1,2, Yingjie Niu^1,2, Qishenng Zuo^1,2, Yani Zhang^1,2, Jiuzhou Song³, Guohong Chen^1,2, Bichun Li^1,2,4

¹Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China,Yangzhou University, ²Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Technology,Yangzhou University, ³Animal & Avian Sciences,University of Maryland, College Park, ⁴College of Biotechnology,Jiangsu University of Science and Technology

Journal (Developmental Biology)

A Guide to Examining Intramuscular Fat Formation and its Cellular Origin in Skeletal Muscle

Connor D. Johnson¹, Lylybell Y. Zhou¹, Daniel Kopinke^1,2

¹Department of Pharmacology and Therapeutics,University of Florida College of Medicine, ²Myology Institute,University of Florida College of Medicine

Journal (Developmental Biology)

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Hemin P. Shah¹, Olivier Devergne¹

¹Department of Biological Sciences,Northern Illinois University

Journal (Developmental Biology)

A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo

Wei Zhang*¹, Xinjuan Lei*¹, Xin Zhou*^2,3, Boling He¹, Liqin Xiao¹, Huimin Yue¹, Shulin Wang¹, Yuting Sun¹, Yajun Wu¹, Liyang Wang^1,4, George Ghartey-Kwansah¹, Odell D. Jones⁵, Joseph L. Bryant⁶, MengMeng Xu⁷, Jianjie Ma³, Xuehon Xu¹

¹National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB,Shaanxi Normal University College of Life Sciences, ²Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine,Xi'an Medical University, ³Ohio State University College of Medicine, ⁴Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center,Harvard Medical School, ⁵University of Pennsylvania ULAR, ⁶University of Maryland School of Medicine, ⁷Columbia University Medical Center

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