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Measuring Proliferation of Vascular Smooth Muscle Cells Using Click Chemistry

Victoria Wong¹, Prakash Arumugam¹, Lucile E. Wrenshall^1,2

¹Department of Neuroscience, Cell Biology, and Physiology,Wright State University, ²Department of Surgery, Boonshoft School of Medicine,Wright State University

Journal (Biology)

Silencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish

Savvas J. Constantinou¹, Linh Nguyen², Frank Kirschbaum², Vielka L. Salazar³, Jason R. Gallant¹

¹Department of Integrative Biology,Michigan State University, ²Faculty of Life Sciences, Unit of Biology and Ecology of Fishes,Humboldt University, ³Department of Biology,Cape Breton University

Journal (Biology)

Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation

Dehia Sahmi-Bounsiar¹, Paulo Victor de Miranda Boratto², Graziele Pereira Oliveira², Jacques Yaacoub Bou Khalil¹, Bernard La Scola^1,2, Julien Andreani²

¹Institut Hospitalo-Universitaire (IHU) - Méditerranée Infection, ²Microbes, Evolution, Phylogeny and Infection (MEΦI), Aix-Marseille Université UM63, Institut de Recherche pour le Développement IRD 198,Assistance Publique - Hôpitaux de Marseille (AP-HM)

Journal (Biology)

Experimental Approach to Examine Leptin Signaling in the Carotid Bodies and its Effects on Control of Breathing

Mi-Kyung Shin¹, Lenise J. Kim¹, Candela Caballero-Eraso², Vsevolod Y. Polotsky¹

¹Department of Medicine,Johns Hopkins University, ²Instituto de Biomedicina de Sevilla (IBiS), Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES),Hospital Universitario Virgen del Rocío/Universidad de Sevilla

Journal (Biology)

Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture

Laurel R. Yohe*^1,2, Paolo Devanna*³, Kalina T.J. Davies⁴, Joshua H.T. Potter⁴, Stephen J. Rossiter⁴, Emma C. Teeling⁵, Sonja C. Vernes*^3,6, Liliana M. Dávalos*^2,7

¹Department of Geology & Geophysics,Yale University, ²Department of Ecology & Evolution,Stony Brook University, ³Neurogenetics of Vocal Communication,Max Planck Institute for Psycholinguistics, ⁴School of Biological and Chemical Sciences,Queen Mary University of London, ⁵School of Biology & Environmental Science,University College Dublin, ⁶Donders Institute for Brain, Cognition and Behavior, ⁷Consortium for Inter-Disciplinary Environmental Research,Stony Brook University

Journal (Biology)

Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization

Bianca Vezzani^1,2, Mario Gomez-Salazar¹, Joan Casamitjana¹, Carlo Tremolada³, Bruno Péault^1,4

¹MRC Center for Regenerative Medicine,University of Edinburgh, ²Dept. of Morphology, Surgery and Experimental Medicine, Section of General Pathology,University of Ferrara, ³Italian Image Institute, ⁴Orthopaedic Hospital Research Center and Broad Stem Cell Research Center, David Geffen School of Medicine,University of California

Journal (Biology)

Isolation of Intact Eyeball to Obtain Integral Ocular Surface Tissue for Histological Examination and Immunohistochemistry

Dianlei Guo¹, Chunqiao Liu¹

¹State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center,Sun Yat-sen University

Journal (Biology)

Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization

Michael L. Neugent*¹, Jashkaran Gadhvi*¹, Kelli L. Palmer¹, Philippe E. Zimmern², Nicole J. De Nisco¹

¹Department of Biological Sciences,University of Texas at Dallas, ²Department of Urology,University of Texas Southwestern Medical Center

Journal (Biology)

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Shailesh K. Shahi¹, Kasra Zarei², Natalya V. Guseva¹, Ashutosh K. Mangalam^1,2,3,4

¹Department of Pathology,University of Iowa, ²Medical Scientist Training Program,University of Iowa, ³Graduate Program in Immunology,University of Iowa, ⁴Graduate Program in Molecular Medicine,University of Iowa

Journal (Biology)

A Simple Approach to Perform TEER Measurements Using a Self-Made Volt-Amperemeter with Programmable Output Frequency

Marianne Theile¹, Linus Wiora¹, Dominik Russ¹, Jonas Reuter¹, Hiroshi Ishikawa², Christian Schwerk³, Horst Schroten³, Stefan Mogk¹

¹Interfaculty Institute of Biochemistry,University of Tübingen, ²Laboratory of Clinical Regenerative Medicine, Department of Neurosurgery, Faculty of Medicine,University of Tsukuba, ³Department of Pediatrics, Medical Faculty Mannheim,Heidelberg University

Journal (Biology)

Standardizing a Non-Lethal Method for Characterizing the Reproductive Status and Larval Development of Freshwater Mussels (Bivalvia: Unionida)

Caitlin E. Beaver¹, Susan R. Geda², Nathan A. Johnson¹

¹Wetland and Aquatic Research Center,United States Geological Survey, ²Garcon Point Aquatic Research Center,Florida Fish and Wildlife Conservation Commission

Journal (Biology)

Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

Yixuan Sun¹, Peipei Zhou¹, Qianhui Zhao¹, Zhefeng Gong¹

¹Department of Neurobiology,Zhejiang University

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