JOVE JOURNAL

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Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography

Brikena Gusek¹, Robert Folk¹, Tala Curry², Mitra Esfandiarei¹

¹Department of Biomedical Sciences, College of Graduate Studies,Midwestern University, ²Department of Child Health, College of Medicine-Phoenix,University of Arizona, Phoenix

Journal (Biology)

CRISPR/Cas9 Gene Editing of Hematopoietic Stem and Progenitor Cells for Gene Therapy Applications

Vigneshwaran Venkatesan*^1,2, Abisha Crystal Christopher*^1,3, Karthik V. Karuppusamy^1,2, Prathibha Babu^1,2, Manoj Kumar K. Alagiri^1,2, Saravanabhavan Thangavel¹

¹Centre for Stem Cell Research (CSCR), A unit of InStem Bengaluru,Christian Medical College campus, ²Manipal Academy of Higher Education, ³Thiruvalluvar University

Journal (Biology)

Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

Deeksha Singh¹, Sara Blicharz¹, Karolina Stefanowicz¹, Laura Ragni², Kornel Michalak³, Agnieszka Bagniewska-Zadworna³, Robert Malinowski¹

¹Integrative Plant Biology Team,Institute of Plant Genetics Polish Academy of Sciences, ²ZMBP-Center for Plant Molecular Biology,University of Tübingen, ³Department of General Botany, Institute of Experimental Biology, Faculty of Biology,Adam Mickiewicz University

Journal (Biology)

Analyzing Starvation-Induced Autophagy in the Drosophila melanogaster Larval Fat Body

Kexin Shi¹, Chao Tong¹

¹Ministry of Education Key Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, Life Sciences Institute,Zhejiang University

Journal (Biology)

In Vivo Vascular Permeability Detection in Mouse Submandibular Gland

Xiangdi Mao¹, Sainan Min², Qihua He³, Xin Cong¹

¹Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, Key Laboratory of Molecular Cardiovascular Sciences,Ministry of Education, and Beijing Key Laboratory of Cardiovascular Receptors Research, ²Department of Oral and Maxillofacial Surgery,Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Reseah Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, ³State Key Laboratory of Natural and Biomimetic Drugs,Peking University

Journal (Biology)

Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain

Florent Peglion¹, Franck Coumailleau¹, Sandrine Etienne-Manneville¹

¹Cell Polarity, Migration and Cancer Unit,Université Paris-Cité, Institut Pasteur, Équipe Labellisée Ligue Contre le Cancer

Journal (Biology)

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

Charles Havnar¹, Kathy Hotzel¹, Carmina Espiritu¹, Amy Lo¹, Joshua D. Webster¹

¹Department of Pathology,Genentech

Journal (Biology)

Viral Transgene Expression in Rodent Hearts and the Assessment of Cardiac Arrhythmia Risk

Aizhu Lu^1,2, Jerry Wang^1,2, Ying Xia^1,2, Ruonan Gu¹, Kyoung-Han Kim^1,2, Erin E. Mulvihill^1,3, Darryl R. Davis^1,2, Rob S. Beanlands¹, Wenbin Liang^1,2

¹University of Ottawa Heart Institute, ²Department of Cellular and Molecular Medicine,University of Ottawa, ³Department of Biochemistry, Microbiology and Immunology,University of Ottawa

Journal (Biology)

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Emilia J. Orzechowska-Licari¹, Joseph F. LaComb¹, Michael Giarrizzo¹, Vincent W. Yang^1,2, Agnieszka B. Bialkowska¹

¹Department of Medicine,Renaissance School of Medicine at Stony Brook University, ²Department of Physiology and Biophysics,Renaissance School of Medicine at Stony Brook University

Journal (Biology)

Self-Nanoemulsification of Healthy Oils to Enhance the Solubility of Lipophilic Drugs

Mubashar Rehman*¹, Muhammad Ziaullah Khan*¹, Muhammad Tayyab*¹, Asadullah Madni², Qandeel Khalid^1,3

¹Department of Pharmacy,Quaid-i-Azam University, ²Department of Pharmaceutics, Faculty of Pharmacy,The Islamia University of Bahawalpur, ³Nazar College of Pharmacy

Journal (Biology)

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Rebecca Lees*¹, Robert Tempest*¹, Alice Law*¹, Dimitri Aubert*¹, Owen G. Davies*², Soraya Williams*², Nick Peake*³, Ben Peacock*¹

¹NanoFCM Co., Ltd, ²School of Sport, Exercise and Health Sciences,Loughborough University, ³Biomolecular Sciences Research Centre,Sheffield Hallam University

Journal (Biology)

Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture

Li Duan^1,2, Xingfu Li^1,2, Xiao Xu^1,2, Limei Xu^1,2, Daping Wang^1,2, Kan Ouyang^1,2, Yujie Liang^1,3

¹Department of Orthopedics,The First affiliated hospital of Shenzhen University, Shenzhen Second People's Hospital, ²Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology,Shenzhen Second People's Hospital, ³Department of Child and Adolescent Psychiatry, Shenzhen Kangning Hospital, Shenzhen Mental Health Center,Shenzhen Key Laboratory for Psychological Healthcare & Shenzhen Institute of Mental Health

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