JOVE JOURNAL

생체공학

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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Marylène Vandevenne*¹, Mathieu Dondelinger*¹, Sami Yunus², Astrid Freischels¹, Régine Freischels¹, Oscar Crasson¹, Noureddine Rhazi¹, Pierre Bogaerts³, Moreno Galleni¹, Patrice Filée⁴

¹Life Science Department,University of Liège, ²Institute of Condensed Matter and Nanoscience,Catholic University of Louvain, ³Laboratory of Clinical Microbiology,Catholic University of Louvain, ⁴Biotechnology Department,CER Group

Journal (Bioengineering)

High Resolution 3D Imaging of the Human Pancreas Neuro-insular Network

Elizabeth Butterworth¹, Wesley Dickerson², Vindhya Vijay³, Kristina Weitzel¹, Julia Cooper¹, Eric W. Atkinson⁴, Jason E. Coleman⁵, Kevin J. Otto⁴, Martha Campbell-Thompson¹

¹Department of Pathology, Immunology and Experimental Medicine,University of Florida, ²Heller School for Social Policy and Management,Brandeis University, ³Department of Medicine, College of Medicine,University of Florida, ⁴Department of Biomedical Engineering, College of Engineering,University of Florida, ⁵Department of Pediatrics, College of Medicine,University of Florida

Journal (Bioengineering)

A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries

Gi-Hun Lee¹, Youngjoon Suh¹, Joong Yull Park¹

¹School of Mechanical Engineering, College of Engineering,Chung-Ang University

Journal (Bioengineering)

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Audrey Bloem¹, Stephanie Rollero², Pauline Seguinot¹, Lucie Crépin³, Marc Perez¹, Christian Picou¹, Carole Camarasa¹

¹UMR SPO, INRA, SupAgroM,Université de Montpellier, ²IWBT Stellenbosch University, ³PILI,Toulouse White Biotechnology

Journal (Bioengineering)

Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation

Ignasi Casanellas^1,2, Anna Lagunas^3,1, Iro Tsintzou¹, Yolanda Vida^4,5, Daniel Collado^4,5, Ezequiel Pérez-Inestrosa^4,5, Cristina Rodríguez-Pereira⁶, Joana Magalhaes^3,6, Pau Gorostiza^1,3,7, José A. Andrades^8,3, José Becerra^8,3,5, Josep Samitier^1,3,2

¹Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), ²Department of Engineering Electronics,University of Barcelona (UB), ³Networking Biomedical Research Center (CIBER), ⁴Instituto de Investigacin Biomédica de Málaga (IBIMA), Department of Organic Chemistry,Universidad de Málaga (UMA), ⁵Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, ⁶Unidad de Bioingeniería Tisular y Terapia Celular (GBTTC-CHUAC), Grupo de Reumatolog ía, Instituto de Investigación Biomèdica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas,Universidade da Coruña (UDC), ⁷Institució Catalana de Recerca i Estudis Avançats (ICREA), ⁸Instituto de Investigación Biomédica de Málaga (IBIMA), Department of Cell Biology, Genetics and Physiology,Universidad de Málaga (UMA)

Journal (Bioengineering)

A Microcontroller Operated Device for the Generation of Liquid Extracts from Conventional Cigarette Smoke and Electronic Cigarette Aerosol

Chastain A. Anderson¹, Rachael E. Bokota¹, Andrew E. Majeste¹, Walter L. Murfee², Shusheng Wang³

¹Cell & Molecular Biology,Tulane University, ²Biomedical Engineering,Tulane University, ³Cell & Molecular Biology & Ophthalmology,Tulane University

Journal (Bioengineering)

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery

Ming J. Cheng¹, Priya Prabakaran¹, Rajiv Kumar^2,3, Srinivas Sridhar^1,2,3, Eno E. Ebong^1,4,5

¹Department of Chemical Engineering,Northeastern University, ²Nanomedicine Science and Technology Center,Northeastern University, ³Department of Physics,Northeastern University, ⁴Departments of Bioengineering,Northeastern University, ⁵Department of Neuroscience,Albert Einstein College of Medicine

Journal (Bioengineering)

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Kritika S. Katiyar*^1,2,3, Carla C. Winter*^1,2,4, Wisberty J. Gordián-Vélez^1,2,4, John C. O'Donnell^1,2, Yeri J. Song^1,5, Nicole S. Hernandez^1,5, Laura A. Struzyna^1,2,4, D. Kacy Cullen^1,2,5

¹Center for Brain Injury & Repair, Department of Neurosurgery, Perelman School of Medicine,University of Pennsylvania, ²Center for Neurotrauma, Neurodegeneration & Restoration,Michael J. Crescenz Veterans Affairs Medical Center, ³School of Biomedical Engineering,Drexel University, ⁴Department of Bioengineering, School of Engineering and Applied Sciences,University of Pennsylvania, ⁵Neuroscience Graduate Group, Perelman School of Medicine,University of Pennsylvania

Journal (Bioengineering)

Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex

Rong Li*¹, Jinxin Miao*^1,2, Zhiqiang Fan¹, SeokHwan Song³, Il-keun Kong^3,4, Yaohe Wang^2,5, Zhongde Wang¹

¹Department of Animal, Dairy, and Veterinary Sciences,Utah State University, ²National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences,Zhengzhou University, ³Department of Animal Science Division of Applied Life Science (BK21 Plus),Gyeongsang National University, ⁴Institute of Agriculture and Life Science,Gyeongsang National University, ⁵Centre for Molecular Oncology, Barts Cancer Institute,Queen Mary University of London

Journal (Bioengineering)

A Protocol for Real-time 3D Single Particle Tracking

Shangguo Hou¹, Kevin Welsher¹

¹Department of Chemistry,Duke University

Journal (Bioengineering)

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

Patrick Scott Thayer¹, Linnea Stridh Orrhult^2,3, Héctor Martínez²

¹CELLINK LLC, ²CELLINK AB, ³3D Bioprinting Centre, Department of Chemistry and Chemical Engineering,Chalmers University of Technology

Journal (Bioengineering)

A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering

Christopher A. Neal¹, Jennifer G. Nelson-Brantley¹, Michael S. Detamore², Hinrich Staecker¹, Adam J. Mellott³

¹Department of Otolaryngology,University of Kansas Medical Center, ²Stephenson School of Biomedical Engineering,University of Oklahoma, ³Department of Plastic Surgery,University of Kansas Medical Center

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