University of Louisville School of Medicine 2 articles published in JoVE Neuroscience An In Vivo Duo-color Method for Imaging Vascular Dynamics Following Contusive Spinal Cord Injury Chen Chen1,2, Yi Ping Zhang3, Yan Sun1,4, Wenhui Xiong1, Lisa B. E. Shields3, Christopher B. Shields3,5, Xiaoming Jin1, Xiao-Ming Xu1 1Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, and Department of Neurological Surgery, Indiana University School of Medicine, 2Program in Medical Neuroscience, Stark Neurosciences Research Institute, Indiana University School of Medicine, 3Norton Neuroscience Institute, Norton Healthcare, 4Department of Human Anatomy & Histoembryology, School of Basic Medical Sciences, Fudan University, 5Department of Neurological Surgery, University of Louisville School of Medicine We introduce an in vivo imaging method using two different fluorescent dyes to track dynamic spinal vascular changes following a contusive spinal cord injury in adult Sprague-Dawley rats. Medicine Intubation-mediated Intratracheal (IMIT) Instillation: A Noninvasive, Lung-specific Delivery System Matthew B Lawrenz1,2, Ramy A. Fodah2, Maria G. Gutierrez2, Jonathan Warawa1,2 1Center for Predictive Medicine for Biodefense and Emerging Infectious Diseases, University of Louisville Medical School, 2Department of Microbiology and Immunology, University of Louisville Medical School Intubation-mediated intratracheal (IMIT) instillation of reagents is an excellent, noninvasive method for studying respiratory disease, as well as a method for instilling therapeutic reagents directly into the lung. It is a rapid and highly reproducible method which is suitable for preclinical testing.