Korea University View Institution's Website 18 articles published in JoVE Biochemistry Arteriovenous Metabolomics to Measure In Vivo Metabolite Exchange in Brown Adipose Tissue Sanghun Lee*1, Gyumin Lim*2,3, Soyeon Kim*1, Hyoju Kim1, Yeon Jin Roh3, Wantae Kim2, Dong Wook Choi3, Su Myung Jung1 1Department of Biological Sciences, Sungkyunkwan University (SKKU), 2Department of Biochemistry, College of Natural Sciences, Chungnam National University, 3Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University In this protocol, methods relevant for BAT-optimized arteriovenous metabolomics using GC-MS in a mouse model are outlined. These methods allow for the acquisition of valuable insights into BAT-mediated metabolite exchange at the organismal level. Neuroscience Electroencephalography Network Indices as Biomarkers of Upper Limb Impairment in Chronic Stroke Ga-Young Choi*1, Won Kee Chang*2, Miseon Shim1, Nuri Kim2, Ji-hee Jang2, Won-Seok Kim2, Han-Jeong Hwang1,3, Nam-Jong Paik2 1Department of Electronics and Information Engineering, Korea University, 2Department of Rehabilitation Medicine, Seoul National University College of Medicine, Seoul National University Bundang Hospital, 3Interdisciplinary Graduate Program for Artificial Intelligence Smart Convergence Technology, Korea University The experimental protocol demonstrates the paradigm for acquiring and analyzing electroencephalography (EEG) signals during upper limb movement in individuals with stroke. The alteration of the functional network of low-beta EEG frequency bands was observed during the movement of the impaired upper limb and was associated with the degree of motor impairment. Biochemistry Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes Dohwan Lee*1, Kyung Wook Wee*1, Hyerin Kim*1, Sung Il Han1, Seungmin Kwak1,2, Dae Sung Yoon3, Jeong Hoon Lee1 1Department of Electrical Engineering, Kwangwoon University, 2Korea Institute of Science and Technology (KIST), 3School of Biomedical Engineering, Korea University Presented is a protocol to fabricate a paper-based device for the effective enrichment and isolation of microvesicles and exosomes. Chemistry Development of Heterogeneous Enantioselective Catalysts using Chiral Metal-Organic Frameworks (MOFs) Jeehwan Han*1, Seongwoo Kim*2, Mi Sun Lee1, Min Kim2, Nakcheol Jeong1 1Department of Chemistry, Korea University, 2Department of Chemistry and BK21Plus Research Team, Chungbuk National University Here, we present a protocol for active site validation of metal-organic framework catalysts by comparing stoichiometric and catalytic carbonyl-ene reactions to find out whether a reaction takes place on the inner or outer surface of metal-organic frameworks. Bioengineering Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration Hwanseok Jang1, Jongseong Kim1, Jennifer H. Shin2, Jeffrey J. Fredberg3, Chan Young Park3, Yongdoo Park1 1Department of Biomedical Sciences, Korea University, 2Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 3Department of Environmental Health, Harvard T.H. Chan School of Public Health Collective cell migration in development, wound healing, and cancer metastasis is often guided by the gradients of growth factors or signaling molecules. Described here is an experimental system combining traction microscopy with a microfluidic system and a demonstration of how to quantify the mechanics of collective migration under biochemical gradient. Biochemistry Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery Arfaxad Reyes-Alcaraz1, Yoo-Na Lee1, Seongsik Yun1, Jong-Ik Hwang1, Jae Young Seong1 1Graduate School of Medicine, Korea University GPCR-β-arrestin interactions are an emerging field in GPCR drug discovery. Accurate, precise and easy to set up methods are necessary to monitor such interactions in living systems. We show a structural complementation assay to monitor GPCR-β-arrestin interactions in real time living cells, and it can be extended to any GPCR. Biochemistry Quantification of Hypopigmentation Activity In Vitro Yeon-Ji Kim1, Min-Jung Kim1, Dong-Keon Kweon2, Seung-Taik Lim1, Sung-Joon Lee1 1Department of Biotechnology, Graduate School of Life Sciences & Biotechnology, College of Life Sciences and Biotechnology, Korea University, 2JinWoo Bio Co. Ltd We describe three experimental methods for evaluating the hypopigmentation activity of chemicals in vitro: quantification of 1) cellular tyrosinase activity and 2) melanin content, and 3) measurement of melanin by cellular melanin staining and image analysis. Bioengineering Syringe-injectable Mesh Electronics for Stable Chronic Rodent Electrophysiology Thomas G. Schuhmann Jr.1, Tao Zhou2, Guosong Hong2, Jung Min Lee2,3, Tian-Ming Fu2, Hong-Gyu Park3, Charles M. Lieber1,2 1John A. Paulson School of Engineering and Applied Sciences, Harvard University, 2Department of Chemistry and Chemical Biology, Harvard University, 3Department of Physics, Korea University Mesh electronics probes seamlessly integrate and provide stable, long-term, single-neuron level recording within the brain. This protocol uses mesh electronics for in vivo experiments, involving the fabrication of mesh electronics, loading into needles, stereotaxic injection, input/output interfacing, recording experiments, and histology of tissue containing mesh probes. Bioengineering Fabrication of Gradient Nanopattern by Thermal Nanoimprinting Technique and Screening of the Response of Human Endothelial Colony-forming Cells Dae Hwan Kim*1, Long-Hui Cui*2, Ha-Rim Seo2, Hyung Joon Joo2, Seung-Cheol Choi2, Do-Sun Lim2, Kyu Back Lee1 1School of Biomedical Engineering, Korea University, 2Department of Cardiology, Korea University Anam Hospital Here, we present a protocol for the fabrication of gradient nanopattern plates via thermal nanoimprinting and the method of screening responses of human endothelial progenitor cells to the nanostructures. By using the described technology, it is possible to produce a scaffold that can manipulate cell behavior by physical stimuli. Immunology and Infection Effects of Exposure of Formaldehyde to a Rat Model of Atopic Dermatitis Induced by Neonatal Capsaicin Treatment Rafael Taeho Han1, Seung Keun Back2, JaeHee Lee1, Hye young Kim1, Hee Jin Kim3, Heung Sik Na1 1Neuroscience Research Institute & Department of Physiology, College of Medicine, Korea University, 2Department of Pharmaceutics and Biotechnology, College of Medical Engineering, Konyang University, 3Division of Biological Science and Technology, Science and Technology College, Yonsei University Wonju Campus We describe here methods for neonatal capsaicin treatment to induce atopic dermatitis in rats and exposure of vaporized formaldehyde to investigate the effects of formaldehyde inhalation on atopic dermatitis. Neuroscience Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors Sungmoo Lee1,2, Hong Hua Piao2, Masoud Sepheri-Rad2, Arong Jung2,3, Uhna Sung2, Yoon-Kyu Song1,4, Bradley J. Baker2 1Department of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, 2Center for Functional Connectomics, Korea Institute of Science and Technology, 3College of Life Sciences and Biotechnology, Korea University, 4Advanced Institutes of Convergence Technology A method for imaging changes in membrane potential using genetically encoded voltage indicators is described. Bioengineering Fabrication of Mechanically Tunable and Bioactive Metal Scaffolds for Biomedical Applications Hyun-Do Jung1, Hyun Lee2, Hyoun-Ee Kim2,3, Young-Hag Koh4, Juha Song3 1Liquid Processing & Casting Technology R&D Group, Korea Institute of Industrial Technology, 2Department of Materials Science and Engineering, Seoul National University, 3Advanced Institutes of Convergence Technology, Seoul National University, 4School of Biomedical Engineering, Korea University Bioactive and mechanically reliable metal scaffolds have been fabricated through a method which consists of two processes, dynamic freeze casting for the fabrication of porous Ti, and coating and densification of the Ti scaffolds. The densification process is simple, effective and applicable to the fabrication of functionally graded scaffolds. Engineering Visible-light Induced Reduction of Graphene Oxide Using Plasmonic Nanoparticle Dinesh Kumar1, Ah-Reum Lee1, Sandeep Kaur2, Dong-Kwon Lim1 1KU-KIST Graduate School of Converging Science and Technology, Korea University, 2Department of BIN Fusion Technology, Chonbuk National University A simple protocol for the preparation of reduced graphene oxide using visible light and plasmonic nanoparticle is described. Developmental Biology A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media Ji-Hye Jung1, Byung Soo Kim1 1Department of Biomedical Science, Graduate School of Medicine, Korea University This protocol provides a simple and efficient way to propagate human pluripotent stem cells (hPSCs) using only conditioned media derived from the human placenta in a gelatin-coated dish without additional exogenous supplementation or hPSC-specific synthetic substrata. Engineering Preparation and Evaluation of Hybrid Composites of Chemical Fuel and Multi-walled Carbon Nanotubes in the Study of Thermopower Waves Hayoung Hwang*1, Taehan Yeo*1, Yonghwan Cho1, Dongjoon Shin1, Wonjoon Choi1 1School of Mechanical Engineering, Korea University A protocol for conducting thermopower wave experiments is presented. The synthesis of hybrid composites of a chemical fuel and micro/nanostructured material, manufacturing of a thermopower wave generator, and methods for measuring the corresponding physical phenomena are described. Neuroscience Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis Syed M.Q. Hussaini*1, Heechul Jun*1, Chang Hoon Cho1, Hyo Jin Kim1, Woon Ryoung Kim2, Mi-Hyeon Jang1,3 1Department of Neurologic Surgery, Mayo Clinic College of Medicine, 2Department of Anatomy, Korea University College of Medicine, 3Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine During adult hippocampal neurogenesis, a distinct set of genetic markers are expressed when a quiescent neural stem cell sequentially progresses and develops into a functionally integrated neuron in the circuit. Using heat-induced antigen retrieval, progenitor cell types that are otherwise difficult to detect are identified with improved effectiveness. Neuroscience MPI CyberMotion Simulator: Implementation of a Novel Motion Simulator to Investigate Multisensory Path Integration in Three Dimensions Michael Barnett-Cowan1, Tobias Meilinger1, Manuel Vidal2, Harald Teufel1, Heinrich H. Bülthoff1,3 1Department of Human Perception, Cognition and Action, Max Planck Institute for Biological Cybernetics, 2Laboratoire de Physiologie de la Perception et de l'Action, Collège de France - CNRS, 3Department of Brain and Cognitive Engineering, Korea University An efficient way to gain insight into how humans navigate themselves in three dimensions is described. The method takes advantage of a motion simulator capable of moving observers in ways unattainable by traditional simulators. Results confirm that movement in the horizontal plane is underestimated, while vertical movement is overestimated. Medicine Use of a Hanging-weight System for Isolated Renal Artery Occlusion Almut Grenz1, Julee H. Hong1, Alexander Badulak1, Douglas Ridyard1, Timothy Luebbert2, Jae-Hwan Kim3, Holger K. Eltzschig1 1Department of Anesthesiology, University of Colorado, 2School of Medicine, University of Colorado, 3Department of Anesthesiology, Korea University College of Medicine A precise murine model for acute kidney injury (AKI) due to ischemia is an important tool to investigate acute kidney injury and possibly find therapeutic tools to treat renal injury. The hanging weight system offers a tool for immediate and reliable renal artery occlusion and reperfusion without causing renal congestion.