1Neuromuscular and Neurogenetic Disorders of Childhood Section, National Institute of Neurological Disorders and Stroke,National Institutes of Health, 2The Dubowitz Neuromuscular Centre, Molecular Neurosciences Section, Developmental Neurosciences Research and Teaching Department, Great Ormond Street Institute of Child Health,University College London, 3Department of Molecular Microbiology and Immunology,University of Southern California, 4NIHR Great Ormond Street Hospital Biomedical Research Centre, 5Genetics and Genomic Medicine Research and Teaching Department, Great Ormond Street Institute of Child Health,University College London
Summary
Qui descriviamo in dettaglio un protocollo ottimizzato per l’iniezione laterale della vena di coda nel topo per somministrare sistemicamente il virus adeno-associato (AAV) nei topi adulti. Inoltre, descriviamo i protocolli dei saggi comunemente usati per valutare la trasduzione di AAV.
