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DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density

DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density

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09:10 min

July 22, 2022

DOI:

09:10 min
July 22, 2022

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Summary

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DetectSyn is an unbiased, rapid fluorescent assay that measures changes in relative synapse (pre- and postsynaptic engagement) number across treatments or disease states. This technique utilizes a proximity ligation technique that can be used both in cultured neurons and fixed tissue.

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