DetectSyn is an unbiased, rapid fluorescent assay that measures changes in relative synapse (pre- and postsynaptic engagement) number across treatments or disease states. This technique utilizes a proximity ligation technique that can be used both in cultured neurons and fixed tissue.
Heaney, C. F., McArdle, C. J., Raab-Graham, K. F. DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density. J. Vis. Exp. (185), e63139, doi:10.3791/63139 (2022).