/
/
DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density
3,016 Views
•
09:10 min
•
July 22, 2022
DOI:
Summary
Automatically generated
DetectSyn is an unbiased, rapid fluorescent assay that measures changes in relative synapse (pre- and postsynaptic engagement) number across treatments or disease states. This technique utilizes a proximity ligation technique that can be used both in cultured neurons and fixed tissue.
Read Article
Cite this Article
Heaney, C. F., McArdle, C. J., Raab-Graham, K. F. DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density. J. Vis. Exp. (185), e63139, doi:10.3791/63139 (2022).
Copy