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DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density
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生化学
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JoVE Journal 生化学
DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density

DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density

3,016 Views

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09:10 min

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July 22, 2022

DOI:

10.3791/63139-v

DOI:
10.3791/63139-v

•

09:10 min
July 22, 2022

•

3 Views
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1Wake Forest Translational Alcohol Research Center,Wake Forest School of Medicine, 2Physiology and Pharmacology,Wake Forest School of Medicine

概要

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DetectSyn is an unbiased, rapid fluorescent assay that measures changes in relative synapse (pre- and postsynaptic engagement) number across treatments or disease states. This technique utilizes a proximity ligation technique that can be used both in cultured neurons and fixed tissue.

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Cite this Article

Heaney, C. F., McArdle, C. J., Raab-Graham, K. F. DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density. J. Vis. Exp. (185), e63139, doi:10.3791/63139 (2022).

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