Journal
/
Developmental Biology
/
Rapid Isolation of Single Cells from Mouse and Human Teeth
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Rapid Isolation of Single Cells from Mouse and Human Teeth

Rapid Isolation of Single Cells from Mouse and Human Teeth

English

Automatically Generated

3,102 Views

•

06:09 min

•

October 28, 2021

DOI:

10.3791/63043-v

DOI:
10.3791/63043-v

•

06:09 min
October 28, 2021

•

5 Views
, , ,
1Department of Histology and Embryology, Faculty of Medicine,Masaryk University, 2Department of Molecular Neuroimmunology, Centre for Brain Research,Medical University of Vienna, 3Department of Physiology and Pharmacology,Karolinska Institute

Summary

Automatically generated

The current protocol presents a fast, efficient, and gentle method for isolating single cells suitable for single-cell RNA-seq analysis from a continuously growing mouse incisor, mouse molar, and human teeth.

Explore More Videos

Single-cell IsolationMouse TeethHuman TeethCell SuspensionDental PulpMandibleEuthanasiaHBSSTweezersScalpelCell Quality

Read Article

Cite this Article

Krivanek, J., Lavicky, J., Bouderlique, T., Adameyko, I. Rapid Isolation of Single Cells from Mouse and Human Teeth. J. Vis. Exp. (176), e63043, doi:10.3791/63043 (2021).

Download .ris file

Copy

Share Video

.

Copy