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Humanized Mediator Release Assay as a Read-Out for Allergen Potency
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

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10:22 min

June 29, 2021

DOI:

10:22 min
June 29, 2021

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Summary

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Here we present the mediator release assay, using a rat basophilic leukemia cell line transfected with the human IgE receptor, to simulate the degranulation of effector cells typically observed in type 1 allergic reactions. This method investigates the biological activity of allergens in a highly sensitive, reproducible, and tailorable manner.

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