Journal
/
/
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

3,120 Views

10:22 min

June 29, 2021

DOI:

10:22 min
June 29, 2021

15 Views
, , ,

Summary

Automatically generated

Here we present the mediator release assay, using a rat basophilic leukemia cell line transfected with the human IgE receptor, to simulate the degranulation of effector cells typically observed in type 1 allergic reactions. This method investigates the biological activity of allergens in a highly sensitive, reproducible, and tailorable manner.

Read Article