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Immunology and Infection
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Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

English

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4,023 Views

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09:02 min

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October 14, 2021

DOI:

10.3791/62670-v

DOI:
10.3791/62670-v

•

09:02 min
October 14, 2021

•

8 Views
, , , , ,
1Department of Obstetrics and Gynaecology, National Institute for Health Research Cambridge Biomedical Research Centre,University of Cambridge School of Clinical Medicine, 2Centre for Trophoblast Research,University of Cambridge

Summary

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This is a method to isolate uterine lymphoid cells from both pregnant and non-pregnant mice. This method can be used for multiple downstream applications such as FACS phenotyping, cell sorting, functional assays, RNA-seq, and proteomics. The protocol here demonstrates how to phenotype group 1 uterine innate lymphoid cells by flow cytometry.

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Cite this Article

Depierreux, D. M., Seshadri, E., Shmeleva, E. V., Kieckbusch, J., Hawkes, D. A., Colucci, F. Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry. J. Vis. Exp. (176), e62670, doi:10.3791/62670 (2021).

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