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Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry
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Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry
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October 14, 2021
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This is a method to isolate uterine lymphoid cells from both pregnant and non-pregnant mice. This method can be used for multiple downstream applications such as FACS phenotyping, cell sorting, functional assays, RNA-seq, and proteomics. The protocol here demonstrates how to phenotype group 1 uterine innate lymphoid cells by flow cytometry.
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Depierreux, D. M., Seshadri, E., Shmeleva, E. V., Kieckbusch, J., Hawkes, D. A., Colucci, F. Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry. J. Vis. Exp. (176), e62670, doi:10.3791/62670 (2021).
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