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Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry
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免疫与感染
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JoVE 杂志 免疫与感染
Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

3,860 Views

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09:02 min

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October 14, 2021

DOI:

10.3791/62670-v

DOI:
10.3791/62670-v

•

09:02 min
October 14, 2021

•

11 Views
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1Department of Obstetrics and Gynaecology, National Institute for Health Research Cambridge Biomedical Research Centre,University of Cambridge School of Clinical Medicine, 2Centre for Trophoblast Research,University of Cambridge

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Uterine Innate Lymphoid CellsFlow CytometryPregnantNon-pregnantLymphocyte IsolationCell ViabilityFetus RemovalEnzymatic DigestionEDTA PBSCell Strainer

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Depierreux, D. M., Seshadri, E., Shmeleva, E. V., Kieckbusch, J., Hawkes, D. A., Colucci, F. Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry. J. Vis. Exp. (176), e62670, doi:10.3791/62670 (2021).

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