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AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
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Neuroscience
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JoVE Journal Neuroscience
AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

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2,411 Views

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09:59 min

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March 31, 2022

DOI:

10.3791/62650-v

DOI:
10.3791/62650-v

•

09:59 min
March 31, 2022

•

3 Views
, ,
1Department of Psychiatry and Behavioral Sciences,University of California, Davis, 2Department of Neurobiology, Physiology and Behavior,University of California, Davis

Summary

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This protocol describes a novel rAAV-based transient enhancer-reporter assay. This assay can be used to induce enhancer-driven expression in vivo in the mouse brain.

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Cite this Article

Warren, T. L., Lambert, J. T., Nord, A. S. AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain. J. Vis. Exp. (181), e62650, doi:10.3791/62650 (2022).

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