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AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
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JoVE 杂志 神经科学
AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

DOI:
10.3791/62650-v

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09:59 min

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March 31, 2022

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, ,
1Department of Psychiatry and Behavioral Sciences,University of California, Davis, 2Department of Neurobiology, Physiology and Behavior,University of California, Davis

Chapters

  • 00:04Introduction
  • 00:39Enhancer Candidate Sequence Cloning into the AAV Vector Plasmid
  • 03:09Intracranially Inject rAAV-Packaged Plasmid into Neonatal Mice
  • 05:32Image and Analyze Brain Tissue Sections for Enhancer Activity
  • 08:10Results: AAV Deployment of Enhancer-Based Expression Constructs in Mouse Brain
  • 09:21Conclusion

Summary

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自动翻译

This protocol describes a novel rAAV-based transient enhancer-reporter assay. This assay can be used to induce enhancer-driven expression in vivo in the mouse brain.

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Keywords: AAVEnhancerExpressionReporterConstructCloningPCRGibson CloningPlasmidVirus TiterIn VivoMouse Brain

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