Rivista
/
Neuroscienze
/
AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

DOI:
10.3791/62650-v

•

09:59 min

•

March 31, 2022

•

, ,
1Department of Psychiatry and Behavioral Sciences,University of California, Davis, 2Department of Neurobiology, Physiology and Behavior,University of California, Davis

Capitoli

  • 00:04Introduction
  • 00:39Enhancer Candidate Sequence Cloning into the AAV Vector Plasmid
  • 03:09Intracranially Inject rAAV-Packaged Plasmid into Neonatal Mice
  • 05:32Image and Analyze Brain Tissue Sections for Enhancer Activity
  • 08:10Results: AAV Deployment of Enhancer-Based Expression Constructs in Mouse Brain
  • 09:21Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes a novel rAAV-based transient enhancer-reporter assay. This assay can be used to induce enhancer-driven expression in vivo in the mouse brain.

Tags

AAVEnhancerExpressionReporterConstructCloningPCRGibson CloningPlasmidVirus TiterIn VivoMouse Brain

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System

Purification of Mouse Brain Vessels

HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

In Vivo Targeted Expression of Optogenetic Proteins Using Silk/AAV Films

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

A Rat Model of EcoHIV Brain Infection

In vivo Calcium Imaging in Mouse Inferior Olive

Read Article