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Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome
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JoVE Journal Developmental Biology
Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome

Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome

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2,898 Views

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09:39 min

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December 04, 2021

DOI:

10.3791/62240-v

DOI:
10.3791/62240-v

•

09:39 min
December 04, 2021

•

8 Views
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1Manipal Institute of Regenerative Medicine,Manipal Academy of Higher Education, Manipal, Karnataka, India-576104, 2Department of Medical Genetics,Manipal Hospitals

Summary

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This protocol describes the generation of integration free iPSCs from fetal tissue fibroblasts through delivery of episomal plasmids by nucleofection followed by description of methods used for iPSC characterization and neuronal differentiation.

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Turner Syndrome45XOFetal CellsInduced Pluripotent Stem CellsIPSCsAneuploidReprogrammingChorionic VilliFibroblastsEpisomal PlasmidsCell CultureDifferentiation

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Cite this Article

Veerasubramanian, N., Karthikeyan, V., Hegde, S., Dhanushkodi, A., Parveen, S. Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome. J. Vis. Exp. (178), e62240, doi:10.3791/62240 (2021).

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