/
/
Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
A subscription to JoVE is required to view this content. Sign in or start your free trial.
3,489 Views
•
10:03 min
•
January 30, 2021
DOI:
1Department of Pathology, Microbiology and Immunology,University of South Carolina School of Medicine, 2Department of Dermatology,Brigham and Women’s Hospital, Harvard Medical School, 3Department of Biochemistry and Molecular Biology,University of Maryland School of Medicine Baltimore, 4Department of Pathology,Albert Einstein College of Medicine, 5Department of Pediatrics,Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy,University of South Carolina
Summary
Automatically generated
Here we describe a protocol for the systematic cultivation of epidermal spheroids in 3D suspension culture. This protocol has wide-ranging applications for use in a variety of epithelial tissue types and for the modeling of several human diseases and conditions.
Read Article
Cite this Article
Woappi, Y., Ezeka, G., Vercellino, J., Bloos, S. M., Creek, K. E., Pirisi, L. Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity. J. Vis. Exp. (167), e62182, doi:10.3791/62182 (2021).
Copy