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Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts
JoVE Journal
Biology
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JoVE Journal Biology
Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts

Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts

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06:57 min

February 06, 2021

DOI:

06:57 min
February 06, 2021

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Summary

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In this protocol, we describe how to generate murine primary epithelial colon monolayers directly from intestinal crypts. We provide experimental approaches to generate confluent monolayers on permeable filters, confluent monolayers for scratch wound healing and biochemical studies, and sparse and confluent monolayers for immunofluorescence analysis.

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