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Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

English

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4,448 Views

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09:36 min

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February 03, 2021

DOI:

10.3791/62148-v

DOI:
10.3791/62148-v

•

09:36 min
February 03, 2021

•

17 Views
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1Department of Bioengineering,Rice University

Summary

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Detailed instructions are provided to build an open-source, modular fluorimeter that is compatible with many low-cost heaters to perform real-time, quantitative isothermal nucleic acid amplification.

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Keyword Extraction:Open-sourceMiniatureFluorimeterIsothermalNucleic Acid AmplificationResource-limited SettingsMolecular DiagnosticsIsothermal AmplificationOptics EnclosureTest BoardFocal Length LensLong Pass FilterEmission FilterDichroic MirrorBeam SplitterExcitation FilterLED

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Coole, J., Kortum, A., Tang, Y., Vohra, I., Maker, Y., Kundrod, K., Natoli, M., Richards-Kortum, R. Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings. J. Vis. Exp. (168), e62148, doi:10.3791/62148 (2021).

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