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Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

DOI:
10.3791/62148-v

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09:36 min

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February 03, 2021

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, , , , , , ,
1Department of Bioengineering,Rice University

Chapitres

  • 00:04Introduction
  • 00:44Optical Housing Assembly
  • 03:39Electronics and Touchscreen Assembly
  • 06:46Real-Time Fluorescence Data Recording
  • 08:08Results: Representative Miniature Fluorimeter Measurements
  • 09:07Conclusion

Summary

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Detailed instructions are provided to build an open-source, modular fluorimeter that is compatible with many low-cost heaters to perform real-time, quantitative isothermal nucleic acid amplification.

Tags

Keyword Extraction:Open-sourceMiniatureFluorimeterIsothermalNucleic Acid AmplificationResource-limited SettingsMolecular DiagnosticsIsothermal AmplificationOptics EnclosureTest BoardFocal Length LensLong Pass FilterEmission FilterDichroic MirrorBeam SplitterExcitation FilterLED

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