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Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
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Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
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February 03, 2021
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Detailed instructions are provided to build an open-source, modular fluorimeter that is compatible with many low-cost heaters to perform real-time, quantitative isothermal nucleic acid amplification.
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Coole, J., Kortum, A., Tang, Y., Vohra, I., Maker, Y., Kundrod, K., Natoli, M., Richards-Kortum, R. Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings. J. Vis. Exp. (168), e62148, doi:10.3791/62148 (2021).
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