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Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes

English

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4,704 Views

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09:58 min

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June 17, 2020

DOI:

10.3791/61348-v

DOI:
10.3791/61348-v

•

09:58 min
June 17, 2020

•

2 Views
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1Department of Ophthalmology, Feinberg School of Medicine,Northwestern University, 2Department of Medicine, Division of Rheumatology, Feinberg School of Medicine,Northwestern University

Summary

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This protocol provides a method to digest whole eyes into a single cell suspension for the purpose of multi-parameter flow cytometric analysis in order to identify specific ocular mononuclear phagocytic populations, including monocytes, microglia, macrophages, and dendritic cells.

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Droho, S., Cuda, C. M., Lavine, J. A. Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes. J. Vis. Exp. (160), e61348, doi:10.3791/61348 (2020).

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